Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
2014
Autori
Milašinović, NikolaKnežević-Jugović, Zorica
Milosavljević, Nedeljko
Lucic-Skorić, Marija
Filipović, Jovanka
Kalagasidis-Krušić, Melina T.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase en...trapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
Izvor:
Biomed research international, 2014, 2014, 64930-Izdavač:
- Hindawi Limited
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Sinteza i karakterizacija novih funkcionalnih polimera i polimernih nanokompozita (RS-MESTD-Basic Research (BR or ON)-172062)
DOI: 10.1155/2014/364930
ISSN: 2314-6133
WoS: 000337421700001
Scopus: 2-s2.0-84902147864
Institucija/grupa
JakovTY - JOUR AU - Milašinović, Nikola AU - Knežević-Jugović, Zorica AU - Milosavljević, Nedeljko AU - Lucic-Skorić, Marija AU - Filipović, Jovanka AU - Kalagasidis-Krušić, Melina T. PY - 2014 UR - http://jakov.kpu.edu.rs/handle/123456789/612 AB - Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well. PB - Hindawi Limited T2 - Biomed research international T1 - Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions VL - 2014 SP - 64930 DO - 10.1155/2014/364930 ER -
@article{ author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lucic-Skorić, Marija and Filipović, Jovanka and Kalagasidis-Krušić, Melina T.", year = "2014", abstract = "Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.", publisher = "Hindawi Limited", journal = "Biomed research international", title = "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions", volume = "2014", pages = "64930", doi = "10.1155/2014/364930" }
Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Lucic-Skorić, M., Filipović, J.,& Kalagasidis-Krušić, M. T.. (2014). Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed research international Hindawi Limited., 2014, 64930. https://doi.org/10.1155/2014/364930
Milašinović N, Knežević-Jugović Z, Milosavljević N, Lucic-Skorić M, Filipović J, Kalagasidis-Krušić MT. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed research international. 2014;2014:64930. doi:10.1155/2014/364930 .
Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lucic-Skorić, Marija, Filipović, Jovanka, Kalagasidis-Krušić, Melina T., "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions" in Biomed research international, 2014 (2014):64930, https://doi.org/10.1155/2014/364930 . .