TY  - CONF
AU  - Pejić, Marija
AU  - Janković, Milica
AU  - Đordjević, Snežana
AU  - Koturević, Biljana
PY  - 2020
UR  - http://jakov.kpu.edu.rs/handle/123456789/1341
AB  - Atropine  from  herbs  has  been  used  in  traditional  medicine  over  centuries,  mainly  because of its hallucinogenic properties. When administrated orally through abusing of Datura stramonium (DS) seeds, atropine is quickly absorbed causing dilatation of the pupil, tachycardia, hyperthermia, dizziness, nausea, extreme confusion, deliriant hallucinations and in some cases death.  Knowing  that  Datura  stramonium  is  one  of  the  “legal  high”  plants  with  a  high  risk  of  toxicity for humans and animals, forensic analysis of such a material is of high importance. This work  aimed  at  developing  a  fast  and  simple  method  for  extraction  and  determination  of  the  atropine content as the main alkaloid in DS seeds. Optimization of parameters for conventional heated solid-liquid extraction of atropine from DS seeds were obtained by varying the particle size  (8.6  mm-1.7  mm),  temperature  (40°C-60  °C),  and  ethanol  concentration  (48.0-96.0  %  v/v). The extracts were analyzed by Acquity UPLC HSS C18 1.8 μm column with mobile phase acetonitrile and formate buffer pH 3.6 (75:25) and flow rate of 0.4 mL/min. Quantitation was done in MRM mode using m/z 290→ 124 and 290→ 93 transitions.
PB  - Belgrade : University of Criminal Investigation and Police Studies
C3  - Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020.
T1  - Extraction and identification of atropine from “legal high” plant species
SP  - 713
EP  - 720
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1341
ER  - 

@conference{
author = "Pejić, Marija and Janković, Milica and Đordjević, Snežana and Koturević, Biljana",
year = "2020",
abstract = "Atropine  from  herbs  has  been  used  in  traditional  medicine  over  centuries,  mainly  because of its hallucinogenic properties. When administrated orally through abusing of Datura stramonium (DS) seeds, atropine is quickly absorbed causing dilatation of the pupil, tachycardia, hyperthermia, dizziness, nausea, extreme confusion, deliriant hallucinations and in some cases death.  Knowing  that  Datura  stramonium  is  one  of  the  “legal  high”  plants  with  a  high  risk  of  toxicity for humans and animals, forensic analysis of such a material is of high importance. This work  aimed  at  developing  a  fast  and  simple  method  for  extraction  and  determination  of  the  atropine content as the main alkaloid in DS seeds. Optimization of parameters for conventional heated solid-liquid extraction of atropine from DS seeds were obtained by varying the particle size  (8.6  mm-1.7  mm),  temperature  (40°C-60  °C),  and  ethanol  concentration  (48.0-96.0  %  v/v). The extracts were analyzed by Acquity UPLC HSS C18 1.8 μm column with mobile phase acetonitrile and formate buffer pH 3.6 (75:25) and flow rate of 0.4 mL/min. Quantitation was done in MRM mode using m/z 290→ 124 and 290→ 93 transitions.",
publisher = "Belgrade : University of Criminal Investigation and Police Studies",
journal = "Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020.",
title = "Extraction and identification of atropine from “legal high” plant species",
pages = "713-720",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1341"
}

Pejić, M., Janković, M., Đordjević, S.,& Koturević, B.. (2020). Extraction and identification of atropine from “legal high” plant species. in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020.
Belgrade : University of Criminal Investigation and Police Studies., 713-720.
https://hdl.handle.net/21.15107/rcub_jakov_1341

Pejić M, Janković M, Đordjević S, Koturević B. Extraction and identification of atropine from “legal high” plant species. in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020.. 2020;:713-720.
https://hdl.handle.net/21.15107/rcub_jakov_1341 .

Pejić, Marija, Janković, Milica, Đordjević, Snežana, Koturević, Biljana, "Extraction and identification of atropine from “legal high” plant species" in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020. (2020):713-720,
https://hdl.handle.net/21.15107/rcub_jakov_1341 .