TY  - JOUR
AU  - Terzić, Ivan
AU  - Ivanović, Jasna
AU  - Zizović, Irena
AU  - Lucić, Skorić, Marija
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Kalagasidis-Krušić, Melina T.
PY  - 2018
UR  - http://jakov.kpu.edu.rs/handle/123456789/833
AB  - Supercritical carbon dioxide (scCO(2)) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with scCO(2) to obtain xero- and aerogels, respectively. Applying 10 min of static and 120 min of dynamic scCO(2) drying at 11 MPa and 45 degrees C followed with the decompression at a rate of 1 MPa/min yielded an advantageous aerogel with favorable swelling kinetics and elasticity, compared to the xerogel and aerogels obtained at other decompression rates and drying times. This aerogel was successfully loaded with thymol (up to 4.6 wt.%) using supercritical scCO(2) at 10 MPa and 35 degrees C. In vitro studies of swelling in PBS at 37 degrees C indicated a great potential of the obtained stimuli-responsive chitosan gels for topical administration of thymol known for antimicrobial, antioxidant and anti-inflammatory activities. POLYM. ENG. SCI., 58:2192-2199, 2018. (c) 2018 Society of Plastics Engineers
PB  - Wiley, Hoboken
T2  - Polymer engineering and science
T1  - A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol
VL  - 58
IS  - 12
SP  - 2192
EP  - 2199
DO  - 10.1002/pen.24834
ER  - 

@article{
author = "Terzić, Ivan and Ivanović, Jasna and Zizović, Irena and Lucić, Skorić, Marija and Milosavljević, Nedeljko and Milašinović, Nikola and Kalagasidis-Krušić, Melina T.",
year = "2018",
abstract = "Supercritical carbon dioxide (scCO(2)) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with scCO(2) to obtain xero- and aerogels, respectively. Applying 10 min of static and 120 min of dynamic scCO(2) drying at 11 MPa and 45 degrees C followed with the decompression at a rate of 1 MPa/min yielded an advantageous aerogel with favorable swelling kinetics and elasticity, compared to the xerogel and aerogels obtained at other decompression rates and drying times. This aerogel was successfully loaded with thymol (up to 4.6 wt.%) using supercritical scCO(2) at 10 MPa and 35 degrees C. In vitro studies of swelling in PBS at 37 degrees C indicated a great potential of the obtained stimuli-responsive chitosan gels for topical administration of thymol known for antimicrobial, antioxidant and anti-inflammatory activities. POLYM. ENG. SCI., 58:2192-2199, 2018. (c) 2018 Society of Plastics Engineers",
publisher = "Wiley, Hoboken",
journal = "Polymer engineering and science",
title = "A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol",
volume = "58",
number = "12",
pages = "2192-2199",
doi = "10.1002/pen.24834"
}

Terzić, I., Ivanović, J., Zizović, I., Lucić, S. M., Milosavljević, N., Milašinović, N.,& Kalagasidis-Krušić, M. T.. (2018). A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol. in Polymer engineering and science
Wiley, Hoboken., 58(12), 2192-2199.
https://doi.org/10.1002/pen.24834

Terzić I, Ivanović J, Zizović I, Lucić SM, Milosavljević N, Milašinović N, Kalagasidis-Krušić MT. A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol. in Polymer engineering and science. 2018;58(12):2192-2199.
doi:10.1002/pen.24834 .

Terzić, Ivan, Ivanović, Jasna, Zizović, Irena, Lucić, Skorić, Marija, Milosavljević, Nedeljko, Milašinović, Nikola, Kalagasidis-Krušić, Melina T., "A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol" in Polymer engineering and science, 58, no. 12 (2018):2192-2199,
https://doi.org/10.1002/pen.24834 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lucic-Skorić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis-Krušić, Melina T.
PY  - 2014
UR  - http://jakov.kpu.edu.rs/handle/123456789/612
AB  - Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
PB  - Hindawi Limited
T2  - Biomed research international
T1  - Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
VL  - 2014
SP  - 64930
DO  - 10.1155/2014/364930
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lucic-Skorić, Marija and Filipović, Jovanka and Kalagasidis-Krušić, Melina T.",
year = "2014",
abstract = "Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.",
publisher = "Hindawi Limited",
journal = "Biomed research international",
title = "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions",
volume = "2014",
pages = "64930",
doi = "10.1155/2014/364930"
}

Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Lucic-Skorić, M., Filipović, J.,& Kalagasidis-Krušić, M. T.. (2014). Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed research international
Hindawi Limited., 2014, 64930.
https://doi.org/10.1155/2014/364930

Milašinović N, Knežević-Jugović Z, Milosavljević N, Lucic-Skorić M, Filipović J, Kalagasidis-Krušić MT. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed research international. 2014;2014:64930.
doi:10.1155/2014/364930 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lucic-Skorić, Marija, Filipović, Jovanka, Kalagasidis-Krušić, Melina T., "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions" in Biomed research international, 2014 (2014):64930,
https://doi.org/10.1155/2014/364930 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Jakovetić, Sonja
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lucić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis-Krušić, Melina T.
PY  - 2014
UR  - http://jakov.kpu.edu.rs/handle/123456789/589
AB  - This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Hindawi Publishing Corp, New York
T2  - Scientific world journal
T1  - Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel
DO  - 10.1155/2014/142123
ER  - 

@article{
author = "Milašinović, Nikola and Jakovetić, Sonja and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lucić, Marija and Filipović, Jovanka and Kalagasidis-Krušić, Melina T.",
year = "2014",
abstract = "This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Hindawi Publishing Corp, New York",
journal = "Scientific world journal",
title = "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel",
doi = "10.1155/2014/142123"
}

Milašinović, N., Jakovetić, S., Knežević-Jugović, Z., Milosavljević, N., Lucić, M., Filipović, J.,& Kalagasidis-Krušić, M. T.. (2014). Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific world journal
Hindawi Publishing Corp, New York..
https://doi.org/10.1155/2014/142123

Milašinović N, Jakovetić S, Knežević-Jugović Z, Milosavljević N, Lucić M, Filipović J, Kalagasidis-Krušić MT. Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific world journal. 2014;.
doi:10.1155/2014/142123 .

Milašinović, Nikola, Jakovetić, Sonja, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lucić, Marija, Filipović, Jovanka, Kalagasidis-Krušić, Melina T., "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel" in Scientific world journal (2014),
https://doi.org/10.1155/2014/142123 . .