TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lucic-Skorić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis-Krušić, Melina T.
PY  - 2014
UR  - http://jakov.kpu.edu.rs/handle/123456789/612
AB  - Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
PB  - Hindawi Limited
T2  - Biomed research international
T1  - Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
VL  - 2014
SP  - 64930
DO  - 10.1155/2014/364930
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lucic-Skorić, Marija and Filipović, Jovanka and Kalagasidis-Krušić, Melina T.",
year = "2014",
abstract = "Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.",
publisher = "Hindawi Limited",
journal = "Biomed research international",
title = "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions",
volume = "2014",
pages = "64930",
doi = "10.1155/2014/364930"
}

Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Lucic-Skorić, M., Filipović, J.,& Kalagasidis-Krušić, M. T.. (2014). Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed research international
Hindawi Limited., 2014, 64930.
https://doi.org/10.1155/2014/364930

Milašinović N, Knežević-Jugović Z, Milosavljević N, Lucic-Skorić M, Filipović J, Kalagasidis-Krušić MT. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed research international. 2014;2014:64930.
doi:10.1155/2014/364930 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lucic-Skorić, Marija, Filipović, Jovanka, Kalagasidis-Krušić, Melina T., "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions" in Biomed research international, 2014 (2014):64930,
https://doi.org/10.1155/2014/364930 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Jakovetić, Sonja
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lucić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis-Krušić, Melina T.
PY  - 2014
UR  - http://jakov.kpu.edu.rs/handle/123456789/589
AB  - This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Hindawi Publishing Corp, New York
T2  - Scientific world journal
T1  - Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel
DO  - 10.1155/2014/142123
ER  - 

@article{
author = "Milašinović, Nikola and Jakovetić, Sonja and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lucić, Marija and Filipović, Jovanka and Kalagasidis-Krušić, Melina T.",
year = "2014",
abstract = "This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Hindawi Publishing Corp, New York",
journal = "Scientific world journal",
title = "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel",
doi = "10.1155/2014/142123"
}

Milašinović, N., Jakovetić, S., Knežević-Jugović, Z., Milosavljević, N., Lucić, M., Filipović, J.,& Kalagasidis-Krušić, M. T.. (2014). Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific world journal
Hindawi Publishing Corp, New York..
https://doi.org/10.1155/2014/142123

Milašinović N, Jakovetić S, Knežević-Jugović Z, Milosavljević N, Lucić M, Filipović J, Kalagasidis-Krušić MT. Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific world journal. 2014;.
doi:10.1155/2014/142123 .

Milašinović, Nikola, Jakovetić, Sonja, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lucić, Marija, Filipović, Jovanka, Kalagasidis-Krušić, Melina T., "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel" in Scientific world journal (2014),
https://doi.org/10.1155/2014/142123 . .