TY  - JOUR
AU  - Zuza, Milena G.
AU  - Milašinović, Nikola
AU  - Jonović, Marko M.
AU  - Jovanović, Jelena R.
AU  - Kalagasidis-Krušić, Melina T.
AU  - Bugarski, Branko M.
AU  - Knežević-Jugović, Zorica
PY  - 2017
UR  - http://jakov.kpu.edu.rs/handle/123456789/772
AB  - In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.
PB  - Springer, New York
T2  - Bioprocess and biosystems engineering
T1  - Design and characterization of alcalase-chitosan conjugates as potential biocatalysts
VL  - 40
IS  - 11
SP  - 1713
EP  - 1723
DO  - 10.1007/s00449-017-1826-7
ER  - 

@article{
author = "Zuza, Milena G. and Milašinović, Nikola and Jonović, Marko M. and Jovanović, Jelena R. and Kalagasidis-Krušić, Melina T. and Bugarski, Branko M. and Knežević-Jugović, Zorica",
year = "2017",
abstract = "In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.",
publisher = "Springer, New York",
journal = "Bioprocess and biosystems engineering",
title = "Design and characterization of alcalase-chitosan conjugates as potential biocatalysts",
volume = "40",
number = "11",
pages = "1713-1723",
doi = "10.1007/s00449-017-1826-7"
}

Zuza, M. G., Milašinović, N., Jonović, M. M., Jovanović, J. R., Kalagasidis-Krušić, M. T., Bugarski, B. M.,& Knežević-Jugović, Z.. (2017). Design and characterization of alcalase-chitosan conjugates as potential biocatalysts. in Bioprocess and biosystems engineering
Springer, New York., 40(11), 1713-1723.
https://doi.org/10.1007/s00449-017-1826-7

Zuza MG, Milašinović N, Jonović MM, Jovanović JR, Kalagasidis-Krušić MT, Bugarski BM, Knežević-Jugović Z. Design and characterization of alcalase-chitosan conjugates as potential biocatalysts. in Bioprocess and biosystems engineering. 2017;40(11):1713-1723.
doi:10.1007/s00449-017-1826-7 .

Zuza, Milena G., Milašinović, Nikola, Jonović, Marko M., Jovanović, Jelena R., Kalagasidis-Krušić, Melina T., Bugarski, Branko M., Knežević-Jugović, Zorica, "Design and characterization of alcalase-chitosan conjugates as potential biocatalysts" in Bioprocess and biosystems engineering, 40, no. 11 (2017):1713-1723,
https://doi.org/10.1007/s00449-017-1826-7 . .

TY  - JOUR
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Kalagasidis-Krušić, Melina T.
AU  - Knežević-Jugović, Zorica
AU  - Savikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko M.
PY  - 2015
UR  - http://jakov.kpu.edu.rs/handle/123456789/639
AB  - Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.
PB  - Sage Publications Ltd, London
T2  - Journal of biomaterials applications
T1  - Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties
VL  - 30
IS  - 5
SP  - 618
EP  - 631
DO  - 10.1177/0885328215598940
ER  - 

@article{
author = "Trifković, Kata T. and Milašinović, Nikola and Đorđević, Verica and Zdunić, Gordana and Kalagasidis-Krušić, Melina T. and Knežević-Jugović, Zorica and Savikin, Katarina and Nedović, Viktor and Bugarski, Branko M.",
year = "2015",
abstract = "Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of biomaterials applications",
title = "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties",
volume = "30",
number = "5",
pages = "618-631",
doi = "10.1177/0885328215598940"
}

Trifković, K. T., Milašinović, N., Đorđević, V., Zdunić, G., Kalagasidis-Krušić, M. T., Knežević-Jugović, Z., Savikin, K., Nedović, V.,& Bugarski, B. M.. (2015). Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of biomaterials applications
Sage Publications Ltd, London., 30(5), 618-631.
https://doi.org/10.1177/0885328215598940

Trifković KT, Milašinović N, Đorđević V, Zdunić G, Kalagasidis-Krušić MT, Knežević-Jugović Z, Savikin K, Nedović V, Bugarski BM. Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of biomaterials applications. 2015;30(5):618-631.
doi:10.1177/0885328215598940 .

Trifković, Kata T., Milašinović, Nikola, Đorđević, Verica, Zdunić, Gordana, Kalagasidis-Krušić, Melina T., Knežević-Jugović, Zorica, Savikin, Katarina, Nedović, Viktor, Bugarski, Branko M., "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties" in Journal of biomaterials applications, 30, no. 5 (2015):618-631,
https://doi.org/10.1177/0885328215598940 . .

TY  - JOUR
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Đorđević, Verica B.
AU  - Kalagasidis-Krušić, Melina T.
AU  - Knežević-Jugović, Zorica
AU  - Nedović, Viktor
AU  - Bugarski, Branko M.
PY  - 2014
UR  - http://jakov.kpu.edu.rs/handle/123456789/577
AB  - In this work chitosan microbeads were prepared by emulsion technique and loaded with thyme polyphenols by diffusion from an external aqueous solution of Thymus serpyllum L. The effects of concentrations of chitosan (1.5-3% (w/v)) and GA (glutaraldehyde) (0.1-0.4% (v/v)), as a crosslinking agent on the main properties of microbeads were assessed. The obtained microgel beads from similar to 220 to similar to 790 mu m in diameter were exposed to controlled drying process at air (at 37 degrees C) after which they contracted to irregular shapes (similar to 70-230 mu m). The loading of dried microbeads with polyphenols was achieved by swelling in the acidic medium. The swelling rate of microbeads decreased with the increase in GA concentration. Upon this rehydration, thyme polypheriols were effectively encapsulated (active load of 66-114mg GAEg(beads)(-1)) and the microbeads recovered a spherical shape. Both, the increase in the amount of the crosslinking agent and the presence of polyphenols, contributed to a more pronounced surface roughness of microbeads. The release of encapsulated polyphenols in simulated gastrointestinal fluids was prolonged to 3 h.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate polymers
T1  - Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols
VL  - 111
SP  - 901
EP  - 907
DO  - 10.1016/j.carbpol.2014.05.053
ER  - 

@article{
author = "Trifković, Kata T. and Milašinović, Nikola and Đorđević, Verica B. and Kalagasidis-Krušić, Melina T. and Knežević-Jugović, Zorica and Nedović, Viktor and Bugarski, Branko M.",
year = "2014",
abstract = "In this work chitosan microbeads were prepared by emulsion technique and loaded with thyme polyphenols by diffusion from an external aqueous solution of Thymus serpyllum L. The effects of concentrations of chitosan (1.5-3% (w/v)) and GA (glutaraldehyde) (0.1-0.4% (v/v)), as a crosslinking agent on the main properties of microbeads were assessed. The obtained microgel beads from similar to 220 to similar to 790 mu m in diameter were exposed to controlled drying process at air (at 37 degrees C) after which they contracted to irregular shapes (similar to 70-230 mu m). The loading of dried microbeads with polyphenols was achieved by swelling in the acidic medium. The swelling rate of microbeads decreased with the increase in GA concentration. Upon this rehydration, thyme polypheriols were effectively encapsulated (active load of 66-114mg GAEg(beads)(-1)) and the microbeads recovered a spherical shape. Both, the increase in the amount of the crosslinking agent and the presence of polyphenols, contributed to a more pronounced surface roughness of microbeads. The release of encapsulated polyphenols in simulated gastrointestinal fluids was prolonged to 3 h.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate polymers",
title = "Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols",
volume = "111",
pages = "901-907",
doi = "10.1016/j.carbpol.2014.05.053"
}

Trifković, K. T., Milašinović, N., Đorđević, V. B., Kalagasidis-Krušić, M. T., Knežević-Jugović, Z., Nedović, V.,& Bugarski, B. M.. (2014). Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols. in Carbohydrate polymers
Elsevier Sci Ltd, Oxford., 111, 901-907.
https://doi.org/10.1016/j.carbpol.2014.05.053

Trifković KT, Milašinović N, Đorđević VB, Kalagasidis-Krušić MT, Knežević-Jugović Z, Nedović V, Bugarski BM. Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols. in Carbohydrate polymers. 2014;111:901-907.
doi:10.1016/j.carbpol.2014.05.053 .

Trifković, Kata T., Milašinović, Nikola, Đorđević, Verica B., Kalagasidis-Krušić, Melina T., Knežević-Jugović, Zorica, Nedović, Viktor, Bugarski, Branko M., "Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols" in Carbohydrate polymers, 111 (2014):901-907,
https://doi.org/10.1016/j.carbpol.2014.05.053 . .