TY  - CONF
AU  - Brajušković, G.
AU  - Branković, A.
AU  - Savić Pavićević, D.
AU  - Mirčetić, J.
AU  - Cerović, S.
AU  - Tomović, S.
AU  - Vuković, I.
AU  - Vukotić, V.
AU  - Romac, S.
PY  - 2011
UR  - http://jakov.kpu.edu.rs/handle/123456789/1553
AB  - Objective: Prostate cancer (PC) is the most common malignant disease in men in the Western Hemisphere. The NOS3 has a role in vascular development, regulation of the vascular tone and tumor growth in PC. In previous studies, the -786 T > C polymorphism was found to be the most important promoter alteration of the NOS3 gene that may affect the PC progression. The purpose of this study was to evaluate 786 T > C, -764A > G, -714 G > T, -690 C > T and -649 G > A polymorphisms in the promoter region of NOS3 gene as genetic indicators of the relative risk of the PC occurrence in Serbian population.

Method: In this study, we characterized these polymorphisms by PCR amplification, followed by capillary electrophoresis sequencing in the peripheral blood samples from 50 patients with PC, 50 benign hyperplasia patients and 50 individuals over 40 years of age who showed no clinical signs of any prostatic disease, that were used as controls.

Results: Three of the analyzed polymorphisms (-764A > G, -714 G > T and -649 G > A) were not detected during this study. It is interesting to observe that when the -786 T > C polymorphism was present, -690 C > T polymorphism was also found.

Conclusion: This study demonstrates no association between the -786 T > C polymorphism in the promoter of the NOS3 gene and the development of PC.
PB  - Helsinki . European Society of Pathology
C3  - Virchows Archiv : European Journal of Pathology (abstracts)
T1  - Correlation between polymorphisms at promoter region of the NOS3 gene and prostate cancer in Serbian population
VL  - 459
IS  - 1 supplement
SP  - S319 (013)
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1553
ER  - 

@conference{
author = "Brajušković, G. and Branković, A. and Savić Pavićević, D. and Mirčetić, J. and Cerović, S. and Tomović, S. and Vuković, I. and Vukotić, V. and Romac, S.",
year = "2011",
abstract = "Objective: Prostate cancer (PC) is the most common malignant disease in men in the Western Hemisphere. The NOS3 has a role in vascular development, regulation of the vascular tone and tumor growth in PC. In previous studies, the -786 T > C polymorphism was found to be the most important promoter alteration of the NOS3 gene that may affect the PC progression. The purpose of this study was to evaluate 786 T > C, -764A > G, -714 G > T, -690 C > T and -649 G > A polymorphisms in the promoter region of NOS3 gene as genetic indicators of the relative risk of the PC occurrence in Serbian population.

Method: In this study, we characterized these polymorphisms by PCR amplification, followed by capillary electrophoresis sequencing in the peripheral blood samples from 50 patients with PC, 50 benign hyperplasia patients and 50 individuals over 40 years of age who showed no clinical signs of any prostatic disease, that were used as controls.

Results: Three of the analyzed polymorphisms (-764A > G, -714 G > T and -649 G > A) were not detected during this study. It is interesting to observe that when the -786 T > C polymorphism was present, -690 C > T polymorphism was also found.

Conclusion: This study demonstrates no association between the -786 T > C polymorphism in the promoter of the NOS3 gene and the development of PC.",
publisher = "Helsinki . European Society of Pathology",
journal = "Virchows Archiv : European Journal of Pathology (abstracts)",
title = "Correlation between polymorphisms at promoter region of the NOS3 gene and prostate cancer in Serbian population",
volume = "459",
number = "1 supplement",
pages = "S319 (013)",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1553"
}

Brajušković, G., Branković, A., Savić Pavićević, D., Mirčetić, J., Cerović, S., Tomović, S., Vuković, I., Vukotić, V.,& Romac, S.. (2011). Correlation between polymorphisms at promoter region of the NOS3 gene and prostate cancer in Serbian population. in Virchows Archiv : European Journal of Pathology (abstracts)
Helsinki . European Society of Pathology., 459(1 supplement), S319 (013).
https://hdl.handle.net/21.15107/rcub_jakov_1553

Brajušković G, Branković A, Savić Pavićević D, Mirčetić J, Cerović S, Tomović S, Vuković I, Vukotić V, Romac S. Correlation between polymorphisms at promoter region of the NOS3 gene and prostate cancer in Serbian population. in Virchows Archiv : European Journal of Pathology (abstracts). 2011;459(1 supplement):S319 (013).
https://hdl.handle.net/21.15107/rcub_jakov_1553 .

Brajušković, G., Branković, A., Savić Pavićević, D., Mirčetić, J., Cerović, S., Tomović, S., Vuković, I., Vukotić, V., Romac, S., "Correlation between polymorphisms at promoter region of the NOS3 gene and prostate cancer in Serbian population" in Virchows Archiv : European Journal of Pathology (abstracts), 459, no. 1 supplement (2011):S319 (013),
https://hdl.handle.net/21.15107/rcub_jakov_1553 .

TY  - CONF
AU  - Brajušković, B.
AU  - Mirković, M.
AU  - Stanković, I.
AU  - Pavićević Savić, D.
AU  - Branković, A.
AU  - Cerović, S.
AU  - Tomović, S.
AU  - Vukotić, V.
AU  - Romac, S.
PY  - 2011
UR  - http://jakov.kpu.edu.rs/handle/123456789/1552
AB  - Introduction & Objectives: Prostate cancer (PC) is the most prevalent type of
cancer in males, comprising about 29% of all malignant tumors. Association with
race, family and specific gene variants suggests strong role of genetics in prostate
cancer etiology. Two nucleotide polymorphisms (SNPs) at 17q12 locus have
been associated with the risk of developing prostate cancer in several previous
genome-wide association studies. The correlation between the prostate cancer
and rs7501939 and rs3760511 has already been confirmed in other populations,
the goal of this study is to test whether it applies to Serbian population.
material & methods: Analyses were done on 150 peripheral blood samples, taken
from 100 patients diagnosed with prostate cancer whose prostate-specific antigen
(PSA) serum levels and Gleason score were available and from 50 patients
diagnosed with benign prostatic hyperplasia (BPH) , while the controls were 100
DNA swab samples taken from healthy individuals. The work proceeded through
PCR amplification of two regions surrounding SNPs, restriction fragment length
polymorphism (RFLP) analysis and random capillary gel electrophoresis of PCR
samples from each SNP group as a control of RFLP analysis. The differences in
genotype frequencies between case and control subjects were tested using a chisquare
test with 1 degree of freedom.
Results:

The Chi-square test was used to determine if there is a statistical
correlation between the SNP and PC and BPH. In the case of rs3760511 there was
no correlation between the controls and the PC, but at the same time, there was a
major statistical correlation between the BPH and PC samples, and also between
BPH and the controls. The rs7501939 also showed no statistical correlation
between the controls and PC samples, but showed correlation between controls
and BPH as well as between BPH and PC samples. Also, there is no statistical
correlation between the Gleason score, PSA levels and the SNP’s studied.
Conclusions: The two SNP’s studied are not correlated to the PC in Serbian
population.
PB  - Elsevier Science
C3  - European Urology Supplements, Abstracts of EAU 7th South Eastern European Meeting (SEEM), and EAU 11th Central European Meeting (CEM)
T1  - Analysis of two single nucleotide polymorphisms at locus 17q12 associated with prostate cancer in Serbian population
VL  - 9
IS  - 10
SP  - 614
EP  - 615 (C8)
DO  - 10.1016/S1569-9056(11)61589-3
ER  - 

@conference{
author = "Brajušković, B. and Mirković, M. and Stanković, I. and Pavićević Savić, D. and Branković, A. and Cerović, S. and Tomović, S. and Vukotić, V. and Romac, S.",
year = "2011",
abstract = "Introduction & Objectives: Prostate cancer (PC) is the most prevalent type of
cancer in males, comprising about 29% of all malignant tumors. Association with
race, family and specific gene variants suggests strong role of genetics in prostate
cancer etiology. Two nucleotide polymorphisms (SNPs) at 17q12 locus have
been associated with the risk of developing prostate cancer in several previous
genome-wide association studies. The correlation between the prostate cancer
and rs7501939 and rs3760511 has already been confirmed in other populations,
the goal of this study is to test whether it applies to Serbian population.
material & methods: Analyses were done on 150 peripheral blood samples, taken
from 100 patients diagnosed with prostate cancer whose prostate-specific antigen
(PSA) serum levels and Gleason score were available and from 50 patients
diagnosed with benign prostatic hyperplasia (BPH) , while the controls were 100
DNA swab samples taken from healthy individuals. The work proceeded through
PCR amplification of two regions surrounding SNPs, restriction fragment length
polymorphism (RFLP) analysis and random capillary gel electrophoresis of PCR
samples from each SNP group as a control of RFLP analysis. The differences in
genotype frequencies between case and control subjects were tested using a chisquare
test with 1 degree of freedom.
Results:

The Chi-square test was used to determine if there is a statistical
correlation between the SNP and PC and BPH. In the case of rs3760511 there was
no correlation between the controls and the PC, but at the same time, there was a
major statistical correlation between the BPH and PC samples, and also between
BPH and the controls. The rs7501939 also showed no statistical correlation
between the controls and PC samples, but showed correlation between controls
and BPH as well as between BPH and PC samples. Also, there is no statistical
correlation between the Gleason score, PSA levels and the SNP’s studied.
Conclusions: The two SNP’s studied are not correlated to the PC in Serbian
population.",
publisher = "Elsevier Science",
journal = "European Urology Supplements, Abstracts of EAU 7th South Eastern European Meeting (SEEM), and EAU 11th Central European Meeting (CEM)",
title = "Analysis of two single nucleotide polymorphisms at locus 17q12 associated with prostate cancer in Serbian population",
volume = "9",
number = "10",
pages = "614-615 (C8)",
doi = "10.1016/S1569-9056(11)61589-3"
}

Brajušković, B., Mirković, M., Stanković, I., Pavićević Savić, D., Branković, A., Cerović, S., Tomović, S., Vukotić, V.,& Romac, S.. (2011). Analysis of two single nucleotide polymorphisms at locus 17q12 associated with prostate cancer in Serbian population. in European Urology Supplements, Abstracts of EAU 7th South Eastern European Meeting (SEEM), and EAU 11th Central European Meeting (CEM)
Elsevier Science., 9(10), 614-615 (C8).
https://doi.org/10.1016/S1569-9056(11)61589-3

Brajušković B, Mirković M, Stanković I, Pavićević Savić D, Branković A, Cerović S, Tomović S, Vukotić V, Romac S. Analysis of two single nucleotide polymorphisms at locus 17q12 associated with prostate cancer in Serbian population. in European Urology Supplements, Abstracts of EAU 7th South Eastern European Meeting (SEEM), and EAU 11th Central European Meeting (CEM). 2011;9(10):614-615 (C8).
doi:10.1016/S1569-9056(11)61589-3 .

Brajušković, B., Mirković, M., Stanković, I., Pavićević Savić, D., Branković, A., Cerović, S., Tomović, S., Vukotić, V., Romac, S., "Analysis of two single nucleotide polymorphisms at locus 17q12 associated with prostate cancer in Serbian population" in European Urology Supplements, Abstracts of EAU 7th South Eastern European Meeting (SEEM), and EAU 11th Central European Meeting (CEM), 9, no. 10 (2011):614-615 (C8),
https://doi.org/10.1016/S1569-9056(11)61589-3 . .