TY  - JOUR
AU  - Day, James R.
AU  - David, Anu
AU  - Kim, Jiwon
AU  - Farkash, Evan A.
AU  - Cascalho, Marilia
AU  - Milašinović, Nikola
AU  - Shikanov, Ariella
PY  - 2018
UR  - http://jakov.kpu.edu.rs/handle/123456789/892
AB  - Poly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.
PB  - Elsevier Sci Ltd, Oxford
T2  - Acta biomaterialia
T1  - The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host
VL  - 67
SP  - 42
EP  - 52
DO  - 10.1016/j.actbio.2017.12.007
ER  - 

@article{
author = "Day, James R. and David, Anu and Kim, Jiwon and Farkash, Evan A. and Cascalho, Marilia and Milašinović, Nikola and Shikanov, Ariella",
year = "2018",
abstract = "Poly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Acta biomaterialia",
title = "The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host",
volume = "67",
pages = "42-52",
doi = "10.1016/j.actbio.2017.12.007"
}

Day, J. R., David, A., Kim, J., Farkash, E. A., Cascalho, M., Milašinović, N.,& Shikanov, A.. (2018). The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host. in Acta biomaterialia
Elsevier Sci Ltd, Oxford., 67, 42-52.
https://doi.org/10.1016/j.actbio.2017.12.007

Day JR, David A, Kim J, Farkash EA, Cascalho M, Milašinović N, Shikanov A. The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host. in Acta biomaterialia. 2018;67:42-52.
doi:10.1016/j.actbio.2017.12.007 .

Day, James R., David, Anu, Kim, Jiwon, Farkash, Evan A., Cascalho, Marilia, Milašinović, Nikola, Shikanov, Ariella, "The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host" in Acta biomaterialia, 67 (2018):42-52,
https://doi.org/10.1016/j.actbio.2017.12.007 . .

TY  - JOUR
AU  - Day, James R.
AU  - David, Anu
AU  - Kim, Jiwon
AU  - Farkash, Evan A.
AU  - Cascalho, Marilia
AU  - Milašinović, Nikola
AU  - Shikanov, Ariella
PY  - 2018
UR  - http://jakov.kpu.edu.rs/handle/123456789/828
AB  - Poly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.
PB  - Elsevier Sci Ltd, Oxford
T2  - Acta biomaterialia
T1  - The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host
VL  - 67
SP  - 42
EP  - 52
DO  - 10.1016/j.actbio.2017.12.007
ER  - 

@article{
author = "Day, James R. and David, Anu and Kim, Jiwon and Farkash, Evan A. and Cascalho, Marilia and Milašinović, Nikola and Shikanov, Ariella",
year = "2018",
abstract = "Poly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Acta biomaterialia",
title = "The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host",
volume = "67",
pages = "42-52",
doi = "10.1016/j.actbio.2017.12.007"
}

Day, J. R., David, A., Kim, J., Farkash, E. A., Cascalho, M., Milašinović, N.,& Shikanov, A.. (2018). The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host. in Acta biomaterialia
Elsevier Sci Ltd, Oxford., 67, 42-52.
https://doi.org/10.1016/j.actbio.2017.12.007

Day JR, David A, Kim J, Farkash EA, Cascalho M, Milašinović N, Shikanov A. The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host. in Acta biomaterialia. 2018;67:42-52.
doi:10.1016/j.actbio.2017.12.007 .

Day, James R., David, Anu, Kim, Jiwon, Farkash, Evan A., Cascalho, Marilia, Milašinović, Nikola, Shikanov, Ariella, "The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host" in Acta biomaterialia, 67 (2018):42-52,
https://doi.org/10.1016/j.actbio.2017.12.007 . .