TY  - CONF
AU  - Vučković, Nemanja
AU  - Glođović, Nikola
AU  - Milašinović, Nikola
PY  - 2023
UR  - http://jakov.kpu.edu.rs/handle/123456789/1506
AB  - Purpose: Polymeric materials are widely used in various industries and fields, such as vehicle and air industry, medicine, pharmacy and nanotechnology, but still insufficiently investigated in forensic applications. Their specific properties could be of a great significance in forensic trace analysis and they could also gratify the cost-benefit requirements. 
Design/Methods/Approach: This paper deals with polyaniline-based polymer powder, obtained by simple precipitating method, with the aim to develop latent fingermarks deposited onto different surfaces, often found at the crime scene. 
Findings: Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FT-IR) analyses confirmed interactions between components of the system. Optical microscopy and scanning electron microscope (SEM) analysis suggested that prepared powder has fine, uniform particles, which easily bind to the sweat and lipid fingerprint residues. Prepared powder was also used to visualize latent fingermarks left on different non-porous, semi-porous and porous surfaces, i.e. plywood, glass and paper. 
Originality/Value: Obtained results showed that prepared powder could be used as a substitution for commercially employed fingerprint powders used in everyday forensic practice/trace analysis.
PB  - Belgrade : University of Criminal Investigation and Police Studies / Beograd : Kriminalističko-policijski univerzitet
C3  - Thematic conference proceedings of international significance : XII International Scientific Conference "Archibald Reiss Days", Belgrade, 8-9 November 2022 / Tematski zbornik radova međunarodnog značaja : XII međunarodni naučni skup ,,Dani Arčibalda Rajsa", Beograd, 8-9. novembra 2022.
T1  - Development of Latent Fingermarks on Different Substrates Using Polyaniline-Based Powder Obtained by Simple Precipitating Method
SP  - 53
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1506
ER  - 

@conference{
author = "Vučković, Nemanja and Glođović, Nikola and Milašinović, Nikola",
year = "2023",
abstract = "Purpose: Polymeric materials are widely used in various industries and fields, such as vehicle and air industry, medicine, pharmacy and nanotechnology, but still insufficiently investigated in forensic applications. Their specific properties could be of a great significance in forensic trace analysis and they could also gratify the cost-benefit requirements. 
Design/Methods/Approach: This paper deals with polyaniline-based polymer powder, obtained by simple precipitating method, with the aim to develop latent fingermarks deposited onto different surfaces, often found at the crime scene. 
Findings: Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FT-IR) analyses confirmed interactions between components of the system. Optical microscopy and scanning electron microscope (SEM) analysis suggested that prepared powder has fine, uniform particles, which easily bind to the sweat and lipid fingerprint residues. Prepared powder was also used to visualize latent fingermarks left on different non-porous, semi-porous and porous surfaces, i.e. plywood, glass and paper. 
Originality/Value: Obtained results showed that prepared powder could be used as a substitution for commercially employed fingerprint powders used in everyday forensic practice/trace analysis.",
publisher = "Belgrade : University of Criminal Investigation and Police Studies / Beograd : Kriminalističko-policijski univerzitet",
journal = "Thematic conference proceedings of international significance : XII International Scientific Conference "Archibald Reiss Days", Belgrade, 8-9 November 2022 / Tematski zbornik radova međunarodnog značaja : XII međunarodni naučni skup ,,Dani Arčibalda Rajsa", Beograd, 8-9. novembra 2022.",
title = "Development of Latent Fingermarks on Different Substrates Using Polyaniline-Based Powder Obtained by Simple Precipitating Method",
pages = "53-64",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1506"
}

Vučković, N., Glođović, N.,& Milašinović, N.. (2023). Development of Latent Fingermarks on Different Substrates Using Polyaniline-Based Powder Obtained by Simple Precipitating Method. in Thematic conference proceedings of international significance : XII International Scientific Conference "Archibald Reiss Days", Belgrade, 8-9 November 2022 / Tematski zbornik radova međunarodnog značaja : XII međunarodni naučni skup ,,Dani Arčibalda Rajsa", Beograd, 8-9. novembra 2022.
Belgrade : University of Criminal Investigation and Police Studies / Beograd : Kriminalističko-policijski univerzitet., 53-64.
https://hdl.handle.net/21.15107/rcub_jakov_1506

Vučković N, Glođović N, Milašinović N. Development of Latent Fingermarks on Different Substrates Using Polyaniline-Based Powder Obtained by Simple Precipitating Method. in Thematic conference proceedings of international significance : XII International Scientific Conference "Archibald Reiss Days", Belgrade, 8-9 November 2022 / Tematski zbornik radova međunarodnog značaja : XII međunarodni naučni skup ,,Dani Arčibalda Rajsa", Beograd, 8-9. novembra 2022.. 2023;:53-64.
https://hdl.handle.net/21.15107/rcub_jakov_1506 .

Vučković, Nemanja, Glođović, Nikola, Milašinović, Nikola, "Development of Latent Fingermarks on Different Substrates Using Polyaniline-Based Powder Obtained by Simple Precipitating Method" in Thematic conference proceedings of international significance : XII International Scientific Conference "Archibald Reiss Days", Belgrade, 8-9 November 2022 / Tematski zbornik radova međunarodnog značaja : XII međunarodni naučni skup ,,Dani Arčibalda Rajsa", Beograd, 8-9. novembra 2022. (2023):53-64,
https://hdl.handle.net/21.15107/rcub_jakov_1506 .

TY  - JOUR
AU  - Vučković, Nemanja
AU  - Koturević, Biljana
AU  - Milašinović, Nikola
PY  - 2022
UR  - http://jakov.kpu.edu.rs/handle/123456789/1504
AB  - The papillary lines are unique for each person, and that feature is frequently used in modern forensic science to identify and distinguish individuals. Fingerprints are often present at the crime scene, but they are usually in the form of latent (invisible) marks which need to be visualized prior to the further processing. Many routinely employed commercial methods show harmful effect on human health, so the research groups are constantly trying to improve or develop some new approaches, and some of them are resorting to the utilization of bio(polymeric) materials. This paper deals with chitosan- and dextran-based biopowders prepared and characterized to evaluate their potential application in visualizing latent fingermarks. Chitosan and dextran are widely used in medicinal and pharmaceutical applications, but there are only a few studies regarding the utilization of these biopolymers as a component for the fingerprint powder. These biopolymeric materials offer many benefits, such as non-toxic properties, specific binding mechanisms, and they also satisfy the cost-benefit demands. Two chitosan-based formulations and one dextran-based formulation were obtained in simple synthesis processes. FT-IR analyses confirmed interactions between components of prepared systems. Optical microscopy showed that prepared powder formulations possess small and uniformly distributed particles, which contributed to their easy binding to the sweat and lipid residues present in the fingerprint trace. Prepared formulations were tested on a rubber (semi-porous) and glass (non-porous) surface. The results indicated that the obtained bio-based powders have the potential to complement commercially exploited physical systems/fingerprint powders in detecting and enhancing latent fingermarks.
PB  - Ankara : Institute of Forensic Sciences
T2  - Turkish Journal of Forensic Science and Crime Studies
T1  - Chitosan And Dextran Based Powders – The Preparation, Performance Comparison and Potential Application in Forensic  Examination of Latent Fingermarks
VL  - 4
IS  - 1
SP  - 3
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1504
ER  - 

@article{
author = "Vučković, Nemanja and Koturević, Biljana and Milašinović, Nikola",
year = "2022",
abstract = "The papillary lines are unique for each person, and that feature is frequently used in modern forensic science to identify and distinguish individuals. Fingerprints are often present at the crime scene, but they are usually in the form of latent (invisible) marks which need to be visualized prior to the further processing. Many routinely employed commercial methods show harmful effect on human health, so the research groups are constantly trying to improve or develop some new approaches, and some of them are resorting to the utilization of bio(polymeric) materials. This paper deals with chitosan- and dextran-based biopowders prepared and characterized to evaluate their potential application in visualizing latent fingermarks. Chitosan and dextran are widely used in medicinal and pharmaceutical applications, but there are only a few studies regarding the utilization of these biopolymers as a component for the fingerprint powder. These biopolymeric materials offer many benefits, such as non-toxic properties, specific binding mechanisms, and they also satisfy the cost-benefit demands. Two chitosan-based formulations and one dextran-based formulation were obtained in simple synthesis processes. FT-IR analyses confirmed interactions between components of prepared systems. Optical microscopy showed that prepared powder formulations possess small and uniformly distributed particles, which contributed to their easy binding to the sweat and lipid residues present in the fingerprint trace. Prepared formulations were tested on a rubber (semi-porous) and glass (non-porous) surface. The results indicated that the obtained bio-based powders have the potential to complement commercially exploited physical systems/fingerprint powders in detecting and enhancing latent fingermarks.",
publisher = "Ankara : Institute of Forensic Sciences",
journal = "Turkish Journal of Forensic Science and Crime Studies",
title = "Chitosan And Dextran Based Powders – The Preparation, Performance Comparison and Potential Application in Forensic  Examination of Latent Fingermarks",
volume = "4",
number = "1",
pages = "3-18",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1504"
}

Vučković, N., Koturević, B.,& Milašinović, N.. (2022). Chitosan And Dextran Based Powders – The Preparation, Performance Comparison and Potential Application in Forensic  Examination of Latent Fingermarks. in Turkish Journal of Forensic Science and Crime Studies
Ankara : Institute of Forensic Sciences., 4(1), 3-18.
https://hdl.handle.net/21.15107/rcub_jakov_1504

Vučković N, Koturević B, Milašinović N. Chitosan And Dextran Based Powders – The Preparation, Performance Comparison and Potential Application in Forensic  Examination of Latent Fingermarks. in Turkish Journal of Forensic Science and Crime Studies. 2022;4(1):3-18.
https://hdl.handle.net/21.15107/rcub_jakov_1504 .

Vučković, Nemanja, Koturević, Biljana, Milašinović, Nikola, "Chitosan And Dextran Based Powders – The Preparation, Performance Comparison and Potential Application in Forensic  Examination of Latent Fingermarks" in Turkish Journal of Forensic Science and Crime Studies, 4, no. 1 (2022):3-18,
https://hdl.handle.net/21.15107/rcub_jakov_1504 .

TY  - CONF
AU  - Vučković, Nemanja
AU  - Koturević, Biljana
AU  - Milašinović, Nikola
PY  - 2021
UR  - http://eskup.kpu.edu.rs/dar/issue/view/6/4
UR  - http://jakov.kpu.edu.rs/handle/123456789/1295
AB  - Biopolymers have numerous advantages, such as their biodegradable, non-toxic,
non-inflammatory and biocompatible properties, and, therefore, have a potential for various
applications. In this paper dextran-based biopolymer powder, obtained from Hydrangea macrophylla
liquid anthocyanins extract by ultrasonic extraction and simple precipitating method,
was synthetized and characterized in order to determine its properties and potential application.
Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FT-IR)
analyses showed interactions between the components of the system. Optical microscopy suggested
that the prepared biopowder formulation was small and somewhat uniform in size, and
also showed its easy binding to the fingerprint residues. Additionally, the prepared biopolymer
powder was used to visualize latent fingerprints left on different non-porous and semi-porous
surfaces, i.e. flat wood, glass, plastic and rubber. The results demonstrated the potential of the
obtained dextran-based biopowder to complement routinely applied systems in developing latent
fingerprints.
PB  - Belgrade : University of Criminal Investigation and Police Studies
C3  - Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 9-10 November 2021
T1  - Characterization and potential application of dextran-based biopolymer powder obtained from Hydrangea macrophylla liquid anthocyanins extract by ultrasonic extraction
SP  - 589
EP  - 598
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1295
ER  - 

@conference{
author = "Vučković, Nemanja and Koturević, Biljana and Milašinović, Nikola",
year = "2021",
abstract = "Biopolymers have numerous advantages, such as their biodegradable, non-toxic,
non-inflammatory and biocompatible properties, and, therefore, have a potential for various
applications. In this paper dextran-based biopolymer powder, obtained from Hydrangea macrophylla
liquid anthocyanins extract by ultrasonic extraction and simple precipitating method,
was synthetized and characterized in order to determine its properties and potential application.
Attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FT-IR)
analyses showed interactions between the components of the system. Optical microscopy suggested
that the prepared biopowder formulation was small and somewhat uniform in size, and
also showed its easy binding to the fingerprint residues. Additionally, the prepared biopolymer
powder was used to visualize latent fingerprints left on different non-porous and semi-porous
surfaces, i.e. flat wood, glass, plastic and rubber. The results demonstrated the potential of the
obtained dextran-based biopowder to complement routinely applied systems in developing latent
fingerprints.",
publisher = "Belgrade : University of Criminal Investigation and Police Studies",
journal = "Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 9-10 November 2021",
title = "Characterization and potential application of dextran-based biopolymer powder obtained from Hydrangea macrophylla liquid anthocyanins extract by ultrasonic extraction",
pages = "589-598",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1295"
}

Vučković, N., Koturević, B.,& Milašinović, N.. (2021). Characterization and potential application of dextran-based biopolymer powder obtained from Hydrangea macrophylla liquid anthocyanins extract by ultrasonic extraction. in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 9-10 November 2021
Belgrade : University of Criminal Investigation and Police Studies., 589-598.
https://hdl.handle.net/21.15107/rcub_jakov_1295

Vučković N, Koturević B, Milašinović N. Characterization and potential application of dextran-based biopolymer powder obtained from Hydrangea macrophylla liquid anthocyanins extract by ultrasonic extraction. in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 9-10 November 2021. 2021;:589-598.
https://hdl.handle.net/21.15107/rcub_jakov_1295 .

Vučković, Nemanja, Koturević, Biljana, Milašinović, Nikola, "Characterization and potential application of dextran-based biopolymer powder obtained from Hydrangea macrophylla liquid anthocyanins extract by ultrasonic extraction" in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 9-10 November 2021 (2021):589-598,
https://hdl.handle.net/21.15107/rcub_jakov_1295 .

TY  - JOUR
AU  - Vučković, Nemanja
AU  - Dimitrijević, Stefan
AU  - Milašinović, Nikola
PY  - 2020
UR  - http://jakov.kpu.edu.rs/handle/123456789/1505
AB  - Fingerprints are usually classified as patent, plastic, and/or latent. However, detection and visualization of latent fingerprints, as relevant traces found at the crime scene are very important for reliable identification of suspects by law enforcement personnel. Chemical and physical methods have now been used for years for that purpose, but they showed many deficiencies, where the most important one was related to their toxicity. Therefore, researchers are in constant search for producing complementary, less harmful methods, and some novel approaches are based on (bio)polymeric materials exploiting their specific properties. In this paper, dextran-based micropowders obtained from anthocyanin solution by the simple precipitating method were synthesized and characterized. Dextran is widely used in medicinal and pharmaceutical applications, but, up to our knowledge, no study has been reported using this biopolymer as a powder component for the developing agent for latent fingermarks visualization. Besides being biodegradable and biocompatible, dextran shows non-toxic properties and thus prevents detrimental effect on humans often present when commercial chemical and physical methods are being routinely employed, while at the same time reduces the overall cost of the obtained powder system. Four different formulations of dextran-based micropowders were prepared with the aim of evaluating their performances in visualizing latent fingerprints. FT-IR analyses confirmed interactions between components of the systems. Optical microscopy showed that prepared powdered samples were small and uniform in size, while at the same time confirmed their easy binding to the sweat and lipid residues present in the latent trace. The results demonstrated that these novel dextran-based powders have the potential to supplement routinely employed physical systems in detecting and visualizing latent fingerprints.
PB  - Ankara : Institute of Forensic Sciences
T2  - Turkish Journal of Forensic Science and Crime Studies
T1  - Visualization of Latent Fingerprints Using Dextran-Based Micropowders Obtained from Anthocyanin Solution
VL  - 2
IS  - 2
SP  - 83
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1505
ER  - 

@article{
author = "Vučković, Nemanja and Dimitrijević, Stefan and Milašinović, Nikola",
year = "2020",
abstract = "Fingerprints are usually classified as patent, plastic, and/or latent. However, detection and visualization of latent fingerprints, as relevant traces found at the crime scene are very important for reliable identification of suspects by law enforcement personnel. Chemical and physical methods have now been used for years for that purpose, but they showed many deficiencies, where the most important one was related to their toxicity. Therefore, researchers are in constant search for producing complementary, less harmful methods, and some novel approaches are based on (bio)polymeric materials exploiting their specific properties. In this paper, dextran-based micropowders obtained from anthocyanin solution by the simple precipitating method were synthesized and characterized. Dextran is widely used in medicinal and pharmaceutical applications, but, up to our knowledge, no study has been reported using this biopolymer as a powder component for the developing agent for latent fingermarks visualization. Besides being biodegradable and biocompatible, dextran shows non-toxic properties and thus prevents detrimental effect on humans often present when commercial chemical and physical methods are being routinely employed, while at the same time reduces the overall cost of the obtained powder system. Four different formulations of dextran-based micropowders were prepared with the aim of evaluating their performances in visualizing latent fingerprints. FT-IR analyses confirmed interactions between components of the systems. Optical microscopy showed that prepared powdered samples were small and uniform in size, while at the same time confirmed their easy binding to the sweat and lipid residues present in the latent trace. The results demonstrated that these novel dextran-based powders have the potential to supplement routinely employed physical systems in detecting and visualizing latent fingerprints.",
publisher = "Ankara : Institute of Forensic Sciences",
journal = "Turkish Journal of Forensic Science and Crime Studies",
title = "Visualization of Latent Fingerprints Using Dextran-Based Micropowders Obtained from Anthocyanin Solution",
volume = "2",
number = "2",
pages = "83-133",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1505"
}

Vučković, N., Dimitrijević, S.,& Milašinović, N.. (2020). Visualization of Latent Fingerprints Using Dextran-Based Micropowders Obtained from Anthocyanin Solution. in Turkish Journal of Forensic Science and Crime Studies
Ankara : Institute of Forensic Sciences., 2(2), 83-133.
https://hdl.handle.net/21.15107/rcub_jakov_1505

Vučković N, Dimitrijević S, Milašinović N. Visualization of Latent Fingerprints Using Dextran-Based Micropowders Obtained from Anthocyanin Solution. in Turkish Journal of Forensic Science and Crime Studies. 2020;2(2):83-133.
https://hdl.handle.net/21.15107/rcub_jakov_1505 .

Vučković, Nemanja, Dimitrijević, Stefan, Milašinović, Nikola, "Visualization of Latent Fingerprints Using Dextran-Based Micropowders Obtained from Anthocyanin Solution" in Turkish Journal of Forensic Science and Crime Studies, 2, no. 2 (2020):83-133,
https://hdl.handle.net/21.15107/rcub_jakov_1505 .

TY  - CONF
AU  - Vučković, Nemanja
AU  - Dimitrijević, Stefan
AU  - Milašinović, Nikola
PY  - 2020
UR  - http://jakov.kpu.edu.rs/handle/123456789/1338
AB  - Dextran is extensively exploited in medicine and pharmacy, but, currently no studies using this biopolymer as a powder system for enhancement of latent fingerprints were published. In  this  paper  four  different  formulations  of  dextran-based  biopolymer  powders,  obtained  by  simple precipitation of dextran within anthocyanin solution, were synthetized and characterized in order to determine potential of these biopowders in forensic application. Since detrimental effect  on  humans  is  often  present  while  routinely  employing  commercial  dusting  methods,  the  main  advantage  of  prepared  dextran-based  biopowders  are  their  non-toxic  properties,  contributing to the safer/healthier operating conditions. The interactions between components of  the  systems  were  confirmed  by  FT-IR  analysis.  Optical  microscopy  was  used  to  determine  the size of the prepared biopowders, while simultaneously confirmed the interaction between powders and the sweat/lipid residues present in the latent trace. The results demonstrated the potential of novel dextran-based biopowders to supplement routinely employed physical systems in visualizing latent fingerprints.
PB  - Belgrade : University of Criminal Investigation and Police Studies
C3  - Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020.
T1  - Detection and enhancement of latent fingerprints using dextran-based biopolymer powders obtained from anthocyanin solution
SP  - 691
EP  - 701
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1338
ER  - 

@conference{
author = "Vučković, Nemanja and Dimitrijević, Stefan and Milašinović, Nikola",
year = "2020",
abstract = "Dextran is extensively exploited in medicine and pharmacy, but, currently no studies using this biopolymer as a powder system for enhancement of latent fingerprints were published. In  this  paper  four  different  formulations  of  dextran-based  biopolymer  powders,  obtained  by  simple precipitation of dextran within anthocyanin solution, were synthetized and characterized in order to determine potential of these biopowders in forensic application. Since detrimental effect  on  humans  is  often  present  while  routinely  employing  commercial  dusting  methods,  the  main  advantage  of  prepared  dextran-based  biopowders  are  their  non-toxic  properties,  contributing to the safer/healthier operating conditions. The interactions between components of  the  systems  were  confirmed  by  FT-IR  analysis.  Optical  microscopy  was  used  to  determine  the size of the prepared biopowders, while simultaneously confirmed the interaction between powders and the sweat/lipid residues present in the latent trace. The results demonstrated the potential of novel dextran-based biopowders to supplement routinely employed physical systems in visualizing latent fingerprints.",
publisher = "Belgrade : University of Criminal Investigation and Police Studies",
journal = "Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020.",
title = "Detection and enhancement of latent fingerprints using dextran-based biopolymer powders obtained from anthocyanin solution",
pages = "691-701",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1338"
}

Vučković, N., Dimitrijević, S.,& Milašinović, N.. (2020). Detection and enhancement of latent fingerprints using dextran-based biopolymer powders obtained from anthocyanin solution. in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020.
Belgrade : University of Criminal Investigation and Police Studies., 691-701.
https://hdl.handle.net/21.15107/rcub_jakov_1338

Vučković N, Dimitrijević S, Milašinović N. Detection and enhancement of latent fingerprints using dextran-based biopolymer powders obtained from anthocyanin solution. in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020.. 2020;:691-701.
https://hdl.handle.net/21.15107/rcub_jakov_1338 .

Vučković, Nemanja, Dimitrijević, Stefan, Milašinović, Nikola, "Detection and enhancement of latent fingerprints using dextran-based biopolymer powders obtained from anthocyanin solution" in Thematic conference proceedings of international significance [Elektronski izvor] / International Scientific Conference "Archibald Reiss Days", Belgrade, 18-19 November 2020. (2020):691-701,
https://hdl.handle.net/21.15107/rcub_jakov_1338 .

TY  - CONF
AU  - Vučković, Nemanja
AU  - Glođović, Nikola
AU  - Milašinović, Nikola
PY  - 2019
UR  - http://jakov.kpu.edu.rs/handle/123456789/1509
AB  - Detection and enhancement of these marks nowadays is of a huge importance for law enforcement agencies in order to identify persons/offenders. Although commercial chemical and physical methods provide satisfying results on multiple surfaces (glass, wood, rubber surface, etc.), many surfaces still pose a great challenge (firearms, colored surfaces and fabrics, etc.), and not to mention their toxicity and detrimental effect to users. Thus, many research groups focus their efforts in constant improvement and development of already used methods and in preparation of new systems. Due to their specific properties, the polymeric materials, especially biopolymers, are drawing an incredible level of the attention, while experiencing the rapid growth in all areas of research and application. However, the application of these materials in the field of forensic science has not yet been sufficiently exploited and the scientific public is almost unaware of its potential in aforementioned forensic application. In this paper, conjugates based on chitosan were obtained by the precipitating method. The initial concentration of the biopolymer (biopolymer/crosslinker ration) was varied and their potential in detection and visualization of latent fingermarks was examined. The prepared micropowders were characterized by spectrophotometric and spectroscopy methods, while scanning electron microscopy confirmed the shape, size and uniformity of the obtained micropowders, as well as their ability to bind to the fingerprint residues. The results demonstrated that these bio-based micropreparations have potential for commercial development and could complement commercial powders in visualizing and enhancing latent fingermarks.
PB  - Ankara : Polis Akademisi
C3  - First International Congress of Forensic Sciences “Multidisciplinary Cooperation in Forensic Sciences: Current Approaches”
T1  - Chitosan-based micropowders for the development of latent fingermarks – the effect of biopolymer concentration on powder properties
SP  - 25
EP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1509
ER  - 

@conference{
author = "Vučković, Nemanja and Glođović, Nikola and Milašinović, Nikola",
year = "2019",
abstract = "Detection and enhancement of these marks nowadays is of a huge importance for law enforcement agencies in order to identify persons/offenders. Although commercial chemical and physical methods provide satisfying results on multiple surfaces (glass, wood, rubber surface, etc.), many surfaces still pose a great challenge (firearms, colored surfaces and fabrics, etc.), and not to mention their toxicity and detrimental effect to users. Thus, many research groups focus their efforts in constant improvement and development of already used methods and in preparation of new systems. Due to their specific properties, the polymeric materials, especially biopolymers, are drawing an incredible level of the attention, while experiencing the rapid growth in all areas of research and application. However, the application of these materials in the field of forensic science has not yet been sufficiently exploited and the scientific public is almost unaware of its potential in aforementioned forensic application. In this paper, conjugates based on chitosan were obtained by the precipitating method. The initial concentration of the biopolymer (biopolymer/crosslinker ration) was varied and their potential in detection and visualization of latent fingermarks was examined. The prepared micropowders were characterized by spectrophotometric and spectroscopy methods, while scanning electron microscopy confirmed the shape, size and uniformity of the obtained micropowders, as well as their ability to bind to the fingerprint residues. The results demonstrated that these bio-based micropreparations have potential for commercial development and could complement commercial powders in visualizing and enhancing latent fingermarks.",
publisher = "Ankara : Polis Akademisi",
journal = "First International Congress of Forensic Sciences “Multidisciplinary Cooperation in Forensic Sciences: Current Approaches”",
title = "Chitosan-based micropowders for the development of latent fingermarks – the effect of biopolymer concentration on powder properties",
pages = "25-38",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1509"
}

Vučković, N., Glođović, N.,& Milašinović, N.. (2019). Chitosan-based micropowders for the development of latent fingermarks – the effect of biopolymer concentration on powder properties. in First International Congress of Forensic Sciences “Multidisciplinary Cooperation in Forensic Sciences: Current Approaches”
Ankara : Polis Akademisi., 25-38.
https://hdl.handle.net/21.15107/rcub_jakov_1509

Vučković N, Glođović N, Milašinović N. Chitosan-based micropowders for the development of latent fingermarks – the effect of biopolymer concentration on powder properties. in First International Congress of Forensic Sciences “Multidisciplinary Cooperation in Forensic Sciences: Current Approaches”. 2019;:25-38.
https://hdl.handle.net/21.15107/rcub_jakov_1509 .

Vučković, Nemanja, Glođović, Nikola, Milašinović, Nikola, "Chitosan-based micropowders for the development of latent fingermarks – the effect of biopolymer concentration on powder properties" in First International Congress of Forensic Sciences “Multidisciplinary Cooperation in Forensic Sciences: Current Approaches” (2019):25-38,
https://hdl.handle.net/21.15107/rcub_jakov_1509 .

TY  - CONF
AU  - Glođović, Nikola
AU  - Vučković, Nemanja
AU  - Milašinović, Nikola
PY  - 2019
UR  - http://jakov.kpu.edu.rs/handle/123456789/1371
AB  - Latent fingermarks are common type of traces found at the crime scene. Therefore, their visualization and enhancement are widely used techniques in identification of offenders and accomplices by law enforcement agencies worldwide. Chemical and physical methods seemed to be reliable for that purpose and have now been used for years. Therefore, researchers focus their work in order to discover new methods for detection and visualization of latent fingermarks. In this regard, the usage of polymeric materials, especially biopolymers, has not yet been sufficiently tested, and scientific public is
almost unaware of the potential of these materials in forensic science. In this paper, conjugates based on chitosan were obtained by the precipitating method and their application in detection and visualization of latent fingermarks was examined. The results demonstrated that this bio-based powder system could be potentially used as substitutes for commercially used powders in
detecting and visualizing of latent fingermarks.
PB  - Belgrade : University of Criminal Investigation and Police Studies
C3  - Thematic conference proceedings of international significance. Vol. 2 / International scientific conference "Archibald Reiss Days", Belgrade, 6-7 November 2019.
T1  - The application of chitosan-based micropowders in development of latent fingermarks
SP  - 427
EP  - 440
UR  - https://hdl.handle.net/21.15107/rcub_jakov_1371
ER  - 

@conference{
author = "Glođović, Nikola and Vučković, Nemanja and Milašinović, Nikola",
year = "2019",
abstract = "Latent fingermarks are common type of traces found at the crime scene. Therefore, their visualization and enhancement are widely used techniques in identification of offenders and accomplices by law enforcement agencies worldwide. Chemical and physical methods seemed to be reliable for that purpose and have now been used for years. Therefore, researchers focus their work in order to discover new methods for detection and visualization of latent fingermarks. In this regard, the usage of polymeric materials, especially biopolymers, has not yet been sufficiently tested, and scientific public is
almost unaware of the potential of these materials in forensic science. In this paper, conjugates based on chitosan were obtained by the precipitating method and their application in detection and visualization of latent fingermarks was examined. The results demonstrated that this bio-based powder system could be potentially used as substitutes for commercially used powders in
detecting and visualizing of latent fingermarks.",
publisher = "Belgrade : University of Criminal Investigation and Police Studies",
journal = "Thematic conference proceedings of international significance. Vol. 2 / International scientific conference "Archibald Reiss Days", Belgrade, 6-7 November 2019.",
title = "The application of chitosan-based micropowders in development of latent fingermarks",
pages = "427-440",
url = "https://hdl.handle.net/21.15107/rcub_jakov_1371"
}

Glođović, N., Vučković, N.,& Milašinović, N.. (2019). The application of chitosan-based micropowders in development of latent fingermarks. in Thematic conference proceedings of international significance. Vol. 2 / International scientific conference "Archibald Reiss Days", Belgrade, 6-7 November 2019.
Belgrade : University of Criminal Investigation and Police Studies., 427-440.
https://hdl.handle.net/21.15107/rcub_jakov_1371

Glođović N, Vučković N, Milašinović N. The application of chitosan-based micropowders in development of latent fingermarks. in Thematic conference proceedings of international significance. Vol. 2 / International scientific conference "Archibald Reiss Days", Belgrade, 6-7 November 2019.. 2019;:427-440.
https://hdl.handle.net/21.15107/rcub_jakov_1371 .

Glođović, Nikola, Vučković, Nemanja, Milašinović, Nikola, "The application of chitosan-based micropowders in development of latent fingermarks" in Thematic conference proceedings of international significance. Vol. 2 / International scientific conference "Archibald Reiss Days", Belgrade, 6-7 November 2019. (2019):427-440,
https://hdl.handle.net/21.15107/rcub_jakov_1371 .

TY  - JOUR
AU  - Day, James R.
AU  - David, Anu
AU  - Kim, Jiwon
AU  - Farkash, Evan A.
AU  - Cascalho, Marilia
AU  - Milašinović, Nikola
AU  - Shikanov, Ariella
PY  - 2018
UR  - http://jakov.kpu.edu.rs/handle/123456789/892
AB  - Poly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.
PB  - Elsevier Sci Ltd, Oxford
T2  - Acta biomaterialia
T1  - The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host
VL  - 67
SP  - 42
EP  - 52
DO  - 10.1016/j.actbio.2017.12.007
ER  - 

@article{
author = "Day, James R. and David, Anu and Kim, Jiwon and Farkash, Evan A. and Cascalho, Marilia and Milašinović, Nikola and Shikanov, Ariella",
year = "2018",
abstract = "Poly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Acta biomaterialia",
title = "The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host",
volume = "67",
pages = "42-52",
doi = "10.1016/j.actbio.2017.12.007"
}

Day, J. R., David, A., Kim, J., Farkash, E. A., Cascalho, M., Milašinović, N.,& Shikanov, A.. (2018). The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host. in Acta biomaterialia
Elsevier Sci Ltd, Oxford., 67, 42-52.
https://doi.org/10.1016/j.actbio.2017.12.007

Day JR, David A, Kim J, Farkash EA, Cascalho M, Milašinović N, Shikanov A. The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host. in Acta biomaterialia. 2018;67:42-52.
doi:10.1016/j.actbio.2017.12.007 .

Day, James R., David, Anu, Kim, Jiwon, Farkash, Evan A., Cascalho, Marilia, Milašinović, Nikola, Shikanov, Ariella, "The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host" in Acta biomaterialia, 67 (2018):42-52,
https://doi.org/10.1016/j.actbio.2017.12.007 . .

TY  - JOUR
AU  - Terzić, Ivan
AU  - Ivanović, Jasna
AU  - Zizović, Irena
AU  - Lucić, Skorić, Marija
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Kalagasidis-Krušić, Melina T.
PY  - 2018
UR  - http://jakov.kpu.edu.rs/handle/123456789/833
AB  - Supercritical carbon dioxide (scCO(2)) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with scCO(2) to obtain xero- and aerogels, respectively. Applying 10 min of static and 120 min of dynamic scCO(2) drying at 11 MPa and 45 degrees C followed with the decompression at a rate of 1 MPa/min yielded an advantageous aerogel with favorable swelling kinetics and elasticity, compared to the xerogel and aerogels obtained at other decompression rates and drying times. This aerogel was successfully loaded with thymol (up to 4.6 wt.%) using supercritical scCO(2) at 10 MPa and 35 degrees C. In vitro studies of swelling in PBS at 37 degrees C indicated a great potential of the obtained stimuli-responsive chitosan gels for topical administration of thymol known for antimicrobial, antioxidant and anti-inflammatory activities. POLYM. ENG. SCI., 58:2192-2199, 2018. (c) 2018 Society of Plastics Engineers
PB  - Wiley, Hoboken
T2  - Polymer engineering and science
T1  - A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol
VL  - 58
IS  - 12
SP  - 2192
EP  - 2199
DO  - 10.1002/pen.24834
ER  - 

@article{
author = "Terzić, Ivan and Ivanović, Jasna and Zizović, Irena and Lucić, Skorić, Marija and Milosavljević, Nedeljko and Milašinović, Nikola and Kalagasidis-Krušić, Melina T.",
year = "2018",
abstract = "Supercritical carbon dioxide (scCO(2)) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with scCO(2) to obtain xero- and aerogels, respectively. Applying 10 min of static and 120 min of dynamic scCO(2) drying at 11 MPa and 45 degrees C followed with the decompression at a rate of 1 MPa/min yielded an advantageous aerogel with favorable swelling kinetics and elasticity, compared to the xerogel and aerogels obtained at other decompression rates and drying times. This aerogel was successfully loaded with thymol (up to 4.6 wt.%) using supercritical scCO(2) at 10 MPa and 35 degrees C. In vitro studies of swelling in PBS at 37 degrees C indicated a great potential of the obtained stimuli-responsive chitosan gels for topical administration of thymol known for antimicrobial, antioxidant and anti-inflammatory activities. POLYM. ENG. SCI., 58:2192-2199, 2018. (c) 2018 Society of Plastics Engineers",
publisher = "Wiley, Hoboken",
journal = "Polymer engineering and science",
title = "A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol",
volume = "58",
number = "12",
pages = "2192-2199",
doi = "10.1002/pen.24834"
}

Terzić, I., Ivanović, J., Zizović, I., Lucić, S. M., Milosavljević, N., Milašinović, N.,& Kalagasidis-Krušić, M. T.. (2018). A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol. in Polymer engineering and science
Wiley, Hoboken., 58(12), 2192-2199.
https://doi.org/10.1002/pen.24834

Terzić I, Ivanović J, Zizović I, Lucić SM, Milosavljević N, Milašinović N, Kalagasidis-Krušić MT. A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol. in Polymer engineering and science. 2018;58(12):2192-2199.
doi:10.1002/pen.24834 .

Terzić, Ivan, Ivanović, Jasna, Zizović, Irena, Lucić, Skorić, Marija, Milosavljević, Nedeljko, Milašinović, Nikola, Kalagasidis-Krušić, Melina T., "A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol" in Polymer engineering and science, 58, no. 12 (2018):2192-2199,
https://doi.org/10.1002/pen.24834 . .

TY  - JOUR
AU  - Day, James R.
AU  - David, Anu
AU  - Kim, Jiwon
AU  - Farkash, Evan A.
AU  - Cascalho, Marilia
AU  - Milašinović, Nikola
AU  - Shikanov, Ariella
PY  - 2018
UR  - http://jakov.kpu.edu.rs/handle/123456789/828
AB  - Poly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.
PB  - Elsevier Sci Ltd, Oxford
T2  - Acta biomaterialia
T1  - The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host
VL  - 67
SP  - 42
EP  - 52
DO  - 10.1016/j.actbio.2017.12.007
ER  - 

@article{
author = "Day, James R. and David, Anu and Kim, Jiwon and Farkash, Evan A. and Cascalho, Marilia and Milašinović, Nikola and Shikanov, Ariella",
year = "2018",
abstract = "Poly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Acta biomaterialia",
title = "The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host",
volume = "67",
pages = "42-52",
doi = "10.1016/j.actbio.2017.12.007"
}

Day, J. R., David, A., Kim, J., Farkash, E. A., Cascalho, M., Milašinović, N.,& Shikanov, A.. (2018). The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host. in Acta biomaterialia
Elsevier Sci Ltd, Oxford., 67, 42-52.
https://doi.org/10.1016/j.actbio.2017.12.007

Day JR, David A, Kim J, Farkash EA, Cascalho M, Milašinović N, Shikanov A. The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host. in Acta biomaterialia. 2018;67:42-52.
doi:10.1016/j.actbio.2017.12.007 .

Day, James R., David, Anu, Kim, Jiwon, Farkash, Evan A., Cascalho, Marilia, Milašinović, Nikola, Shikanov, Ariella, "The impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the host" in Acta biomaterialia, 67 (2018):42-52,
https://doi.org/10.1016/j.actbio.2017.12.007 . .

TY  - JOUR
AU  - Zuza, Milena G.
AU  - Milašinović, Nikola
AU  - Jonović, Marko M.
AU  - Jovanović, Jelena R.
AU  - Kalagasidis-Krušić, Melina T.
AU  - Bugarski, Branko M.
AU  - Knežević-Jugović, Zorica
PY  - 2017
UR  - http://jakov.kpu.edu.rs/handle/123456789/772
AB  - In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.
PB  - Springer, New York
T2  - Bioprocess and biosystems engineering
T1  - Design and characterization of alcalase-chitosan conjugates as potential biocatalysts
VL  - 40
IS  - 11
SP  - 1713
EP  - 1723
DO  - 10.1007/s00449-017-1826-7
ER  - 

@article{
author = "Zuza, Milena G. and Milašinović, Nikola and Jonović, Marko M. and Jovanović, Jelena R. and Kalagasidis-Krušić, Melina T. and Bugarski, Branko M. and Knežević-Jugović, Zorica",
year = "2017",
abstract = "In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.",
publisher = "Springer, New York",
journal = "Bioprocess and biosystems engineering",
title = "Design and characterization of alcalase-chitosan conjugates as potential biocatalysts",
volume = "40",
number = "11",
pages = "1713-1723",
doi = "10.1007/s00449-017-1826-7"
}

Zuza, M. G., Milašinović, N., Jonović, M. M., Jovanović, J. R., Kalagasidis-Krušić, M. T., Bugarski, B. M.,& Knežević-Jugović, Z.. (2017). Design and characterization of alcalase-chitosan conjugates as potential biocatalysts. in Bioprocess and biosystems engineering
Springer, New York., 40(11), 1713-1723.
https://doi.org/10.1007/s00449-017-1826-7

Zuza MG, Milašinović N, Jonović MM, Jovanović JR, Kalagasidis-Krušić MT, Bugarski BM, Knežević-Jugović Z. Design and characterization of alcalase-chitosan conjugates as potential biocatalysts. in Bioprocess and biosystems engineering. 2017;40(11):1713-1723.
doi:10.1007/s00449-017-1826-7 .

Zuza, Milena G., Milašinović, Nikola, Jonović, Marko M., Jovanović, Jelena R., Kalagasidis-Krušić, Melina T., Bugarski, Branko M., Knežević-Jugović, Zorica, "Design and characterization of alcalase-chitosan conjugates as potential biocatalysts" in Bioprocess and biosystems engineering, 40, no. 11 (2017):1713-1723,
https://doi.org/10.1007/s00449-017-1826-7 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Calija, Bojan
AU  - Vidović, Bojana
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - http://jakov.kpu.edu.rs/handle/123456789/735
AB  - The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of the Taiwan institute of chemical engineers
T1  - Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method
VL  - 60
SP  - 106
EP  - 112
DO  - 10.1016/j.jtice.2015.10.037
ER  - 

@article{
author = "Milašinović, Nikola and Calija, Bojan and Vidović, Bojana and Crevar-Sakač, Milkica and Vujić, Zorica and Knežević-Jugović, Zorica",
year = "2016",
abstract = "The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of the Taiwan institute of chemical engineers",
title = "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method",
volume = "60",
pages = "106-112",
doi = "10.1016/j.jtice.2015.10.037"
}

Milašinović, N., Calija, B., Vidović, B., Crevar-Sakač, M., Vujić, Z.,& Knežević-Jugović, Z.. (2016). Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan institute of chemical engineers
Elsevier Science Bv, Amsterdam., 60, 106-112.
https://doi.org/10.1016/j.jtice.2015.10.037

Milašinović N, Calija B, Vidović B, Crevar-Sakač M, Vujić Z, Knežević-Jugović Z. Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan institute of chemical engineers. 2016;60:106-112.
doi:10.1016/j.jtice.2015.10.037 .

Milašinović, Nikola, Calija, Bojan, Vidović, Bojana, Crevar-Sakač, Milkica, Vujić, Zorica, Knežević-Jugović, Zorica, "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method" in Journal of the Taiwan institute of chemical engineers, 60 (2016):106-112,
https://doi.org/10.1016/j.jtice.2015.10.037 . .

TY  - JOUR
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Kotur-Stevuljević, Jelena
AU  - Dilber, Sanda P.
AU  - Kalagasidis-Krušić, Melina T.
AU  - Đoržević, Brižita
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - http://jakov.kpu.edu.rs/handle/123456789/732
AB  - Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity
VL  - 70
IS  - 1
SP  - 49
EP  - 58
DO  - 10.2298/HEMIND141119010V
ER  - 

@article{
author = "Vidović, Bojana and Milašinović, Nikola and Kotur-Stevuljević, Jelena and Dilber, Sanda P. and Kalagasidis-Krušić, Melina T. and Đoržević, Brižita and Knežević-Jugović, Zorica",
year = "2016",
abstract = "Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity",
volume = "70",
number = "1",
pages = "49-58",
doi = "10.2298/HEMIND141119010V"
}

Vidović, B., Milašinović, N., Kotur-Stevuljević, J., Dilber, S. P., Kalagasidis-Krušić, M. T., Đoržević, B.,& Knežević-Jugović, Z.. (2016). Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija
Association of Chemical Engineers of Serbia., 70(1), 49-58.
https://doi.org/10.2298/HEMIND141119010V

Vidović B, Milašinović N, Kotur-Stevuljević J, Dilber SP, Kalagasidis-Krušić MT, Đoržević B, Knežević-Jugović Z. Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija. 2016;70(1):49-58.
doi:10.2298/HEMIND141119010V .

Vidović, Bojana, Milašinović, Nikola, Kotur-Stevuljević, Jelena, Dilber, Sanda P., Kalagasidis-Krušić, Melina T., Đoržević, Brižita, Knežević-Jugović, Zorica, "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity" in Hemijska industrija, 70, no. 1 (2016):49-58,
https://doi.org/10.2298/HEMIND141119010V . .

TY  - JOUR
AU  - Milašinović, Nikola
PY  - 2016
UR  - http://jakov.kpu.edu.rs/handle/123456789/714
AB  - The identification of latent fingerprints is crucial for the security agencies in order to identify persons suspected of having committed criminal acts. Latent prints often remain as random impressions onto the surfaces of various objects. Huge progress has been made in the identification processes using rolled and plain impressions fingerprints, while detection and comparison of latent prints still remains a challenging task. In addition to the existing standardized methods, in this paper the possible utilization of natural and synthetic polymers and their conjugates with the same goal will be reviewed, by means of latent fingerprints detection and development.
AB  - Identifikacija latentnih otisaka papilarnih linija od ključne je važnosti za bezbednosne službe u identifikaciji osoba osumnjičenih za izvršenje kriminalnih dela. Latentni otisci ostaju kao slučajni utisci na površinama najrazličitijih objekata. Ogroman napredak je postignut u identifikaciji pomoću valjanih otisaka prstiju, dok upoređivanje latentnih otisaka ostaje izazov. Pored postojećih, kriminalističkim tehničarima poznatih standardizovanih metoda za otkrivanje i razvijanje latentnih tragova otisaka papilarnih linija, u radu je dat pregled mogućnosti upotrebe prirodnih i sintetskih polimera i njihovih konjugata. Novije metode detekcije uključuju primenu savremenih (nano)materijala, nanokristala i nanokaiševa za razvijanje latentnih otisaka prstiju, sa visokom osetljivošću, dobrim kontrastom, visokom selektivnošću i efikasnošću i niskim pozadinskim šumom, na različitim podlogama. Sinteza i primena svih opisanih materijala zahtevaju dalje poboljšanje performansi već postojećih sistema za detekciju latentnih otisaka papilrnih linija, ali i otvaraju mogućnosti sinteze potpuno novih takvih sistema.
PB  - Kriminalističko- policijska akademija, Beograd
T2  - Nauka, bezbednost, policija
T1  - Polymers in criminalistics: Latent fingerprint detection and enhancement: From idea to practical application
T1  - Polimeri u kriminalistici - otkrivanje latentnih tragova otisaka prstiju - od ideje do praktične primene
VL  - 21
IS  - 3
SP  - 133
EP  - 148
DO  - 10.5937/nabepo21-11888
ER  - 

@article{
author = "Milašinović, Nikola",
year = "2016",
abstract = "The identification of latent fingerprints is crucial for the security agencies in order to identify persons suspected of having committed criminal acts. Latent prints often remain as random impressions onto the surfaces of various objects. Huge progress has been made in the identification processes using rolled and plain impressions fingerprints, while detection and comparison of latent prints still remains a challenging task. In addition to the existing standardized methods, in this paper the possible utilization of natural and synthetic polymers and their conjugates with the same goal will be reviewed, by means of latent fingerprints detection and development., Identifikacija latentnih otisaka papilarnih linija od ključne je važnosti za bezbednosne službe u identifikaciji osoba osumnjičenih za izvršenje kriminalnih dela. Latentni otisci ostaju kao slučajni utisci na površinama najrazličitijih objekata. Ogroman napredak je postignut u identifikaciji pomoću valjanih otisaka prstiju, dok upoređivanje latentnih otisaka ostaje izazov. Pored postojećih, kriminalističkim tehničarima poznatih standardizovanih metoda za otkrivanje i razvijanje latentnih tragova otisaka papilarnih linija, u radu je dat pregled mogućnosti upotrebe prirodnih i sintetskih polimera i njihovih konjugata. Novije metode detekcije uključuju primenu savremenih (nano)materijala, nanokristala i nanokaiševa za razvijanje latentnih otisaka prstiju, sa visokom osetljivošću, dobrim kontrastom, visokom selektivnošću i efikasnošću i niskim pozadinskim šumom, na različitim podlogama. Sinteza i primena svih opisanih materijala zahtevaju dalje poboljšanje performansi već postojećih sistema za detekciju latentnih otisaka papilrnih linija, ali i otvaraju mogućnosti sinteze potpuno novih takvih sistema.",
publisher = "Kriminalističko- policijska akademija, Beograd",
journal = "Nauka, bezbednost, policija",
title = "Polymers in criminalistics: Latent fingerprint detection and enhancement: From idea to practical application, Polimeri u kriminalistici - otkrivanje latentnih tragova otisaka prstiju - od ideje do praktične primene",
volume = "21",
number = "3",
pages = "133-148",
doi = "10.5937/nabepo21-11888"
}

Milašinović, N.. (2016). Polymers in criminalistics: Latent fingerprint detection and enhancement: From idea to practical application. in Nauka, bezbednost, policija
Kriminalističko- policijska akademija, Beograd., 21(3), 133-148.
https://doi.org/10.5937/nabepo21-11888

Milašinović N. Polymers in criminalistics: Latent fingerprint detection and enhancement: From idea to practical application. in Nauka, bezbednost, policija. 2016;21(3):133-148.
doi:10.5937/nabepo21-11888 .

Milašinović, Nikola, "Polymers in criminalistics: Latent fingerprint detection and enhancement: From idea to practical application" in Nauka, bezbednost, policija, 21, no. 3 (2016):133-148,
https://doi.org/10.5937/nabepo21-11888 . .

TY  - CONF
AU  - Vidović, Bojana
AU  - Dodevska, Margarita
AU  - Milašinović, Nikola
AU  - Cakar, Uros
AU  - Todorović, Vanja
AU  - Stanković, Ivan
AU  - Đoržević, Brižita
PY  - 2015
UR  - http://jakov.kpu.edu.rs/handle/123456789/673
PB  - Karger, Basel
C3  - Annals of nutrition and metabolism
T1  - Dietary total antioxidant capacity and polyphenols intake in Serbian university students
VL  - 67
SP  - 439
EP  - 439
UR  - https://hdl.handle.net/21.15107/rcub_jakov_673
ER  - 

@conference{
author = "Vidović, Bojana and Dodevska, Margarita and Milašinović, Nikola and Cakar, Uros and Todorović, Vanja and Stanković, Ivan and Đoržević, Brižita",
year = "2015",
publisher = "Karger, Basel",
journal = "Annals of nutrition and metabolism",
title = "Dietary total antioxidant capacity and polyphenols intake in Serbian university students",
volume = "67",
pages = "439-439",
url = "https://hdl.handle.net/21.15107/rcub_jakov_673"
}

Vidović, B., Dodevska, M., Milašinović, N., Cakar, U., Todorović, V., Stanković, I.,& Đoržević, B.. (2015). Dietary total antioxidant capacity and polyphenols intake in Serbian university students. in Annals of nutrition and metabolism
Karger, Basel., 67, 439-439.
https://hdl.handle.net/21.15107/rcub_jakov_673

Vidović B, Dodevska M, Milašinović N, Cakar U, Todorović V, Stanković I, Đoržević B. Dietary total antioxidant capacity and polyphenols intake in Serbian university students. in Annals of nutrition and metabolism. 2015;67:439-439.
https://hdl.handle.net/21.15107/rcub_jakov_673 .

Vidović, Bojana, Dodevska, Margarita, Milašinović, Nikola, Cakar, Uros, Todorović, Vanja, Stanković, Ivan, Đoržević, Brižita, "Dietary total antioxidant capacity and polyphenols intake in Serbian university students" in Annals of nutrition and metabolism, 67 (2015):439-439,
https://hdl.handle.net/21.15107/rcub_jakov_673 .

TY  - CONF
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Vidović, Jana
AU  - Calija, Bojan
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Milić, Jela
AU  - Đoržević, Brižita
AU  - Kalagasidis-Krušić, Melina T.
AU  - Knežević-Jugović, Zorica
PY  - 2015
UR  - http://jakov.kpu.edu.rs/handle/123456789/672
PB  - Karger, Basel
C3  - Annals of nutrition and metabolism
T1  - Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity
VL  - 67
SP  - 484
EP  - 485
UR  - https://hdl.handle.net/21.15107/rcub_jakov_672
ER  - 

@conference{
author = "Vidović, Bojana and Milašinović, Nikola and Vidović, Jana and Calija, Bojan and Crevar-Sakač, Milkica and Vujić, Zorica and Milić, Jela and Đoržević, Brižita and Kalagasidis-Krušić, Melina T. and Knežević-Jugović, Zorica",
year = "2015",
publisher = "Karger, Basel",
journal = "Annals of nutrition and metabolism",
title = "Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity",
volume = "67",
pages = "484-485",
url = "https://hdl.handle.net/21.15107/rcub_jakov_672"
}

Vidović, B., Milašinović, N., Vidović, J., Calija, B., Crevar-Sakač, M., Vujić, Z., Milić, J., Đoržević, B., Kalagasidis-Krušić, M. T.,& Knežević-Jugović, Z.. (2015). Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity. in Annals of nutrition and metabolism
Karger, Basel., 67, 484-485.
https://hdl.handle.net/21.15107/rcub_jakov_672

Vidović B, Milašinović N, Vidović J, Calija B, Crevar-Sakač M, Vujić Z, Milić J, Đoržević B, Kalagasidis-Krušić MT, Knežević-Jugović Z. Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity. in Annals of nutrition and metabolism. 2015;67:484-485.
https://hdl.handle.net/21.15107/rcub_jakov_672 .

Vidović, Bojana, Milašinović, Nikola, Vidović, Jana, Calija, Bojan, Crevar-Sakač, Milkica, Vujić, Zorica, Milić, Jela, Đoržević, Brižita, Kalagasidis-Krušić, Melina T., Knežević-Jugović, Zorica, "Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity" in Annals of nutrition and metabolism, 67 (2015):484-485,
https://hdl.handle.net/21.15107/rcub_jakov_672 .

TY  - JOUR
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Kalagasidis-Krušić, Melina T.
AU  - Knežević-Jugović, Zorica
AU  - Savikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko M.
PY  - 2015
UR  - http://jakov.kpu.edu.rs/handle/123456789/639
AB  - Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.
PB  - Sage Publications Ltd, London
T2  - Journal of biomaterials applications
T1  - Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties
VL  - 30
IS  - 5
SP  - 618
EP  - 631
DO  - 10.1177/0885328215598940
ER  - 

@article{
author = "Trifković, Kata T. and Milašinović, Nikola and Đorđević, Verica and Zdunić, Gordana and Kalagasidis-Krušić, Melina T. and Knežević-Jugović, Zorica and Savikin, Katarina and Nedović, Viktor and Bugarski, Branko M.",
year = "2015",
abstract = "Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of biomaterials applications",
title = "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties",
volume = "30",
number = "5",
pages = "618-631",
doi = "10.1177/0885328215598940"
}

Trifković, K. T., Milašinović, N., Đorđević, V., Zdunić, G., Kalagasidis-Krušić, M. T., Knežević-Jugović, Z., Savikin, K., Nedović, V.,& Bugarski, B. M.. (2015). Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of biomaterials applications
Sage Publications Ltd, London., 30(5), 618-631.
https://doi.org/10.1177/0885328215598940

Trifković KT, Milašinović N, Đorđević V, Zdunić G, Kalagasidis-Krušić MT, Knežević-Jugović Z, Savikin K, Nedović V, Bugarski BM. Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of biomaterials applications. 2015;30(5):618-631.
doi:10.1177/0885328215598940 .

Trifković, Kata T., Milašinović, Nikola, Đorđević, Verica, Zdunić, Gordana, Kalagasidis-Krušić, Melina T., Knežević-Jugović, Zorica, Savikin, Katarina, Nedović, Viktor, Bugarski, Branko M., "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties" in Journal of biomaterials applications, 30, no. 5 (2015):618-631,
https://doi.org/10.1177/0885328215598940 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lucic-Skorić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis-Krušić, Melina T.
PY  - 2014
UR  - http://jakov.kpu.edu.rs/handle/123456789/612
AB  - Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
PB  - Hindawi Limited
T2  - Biomed research international
T1  - Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
VL  - 2014
SP  - 64930
DO  - 10.1155/2014/364930
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lucic-Skorić, Marija and Filipović, Jovanka and Kalagasidis-Krušić, Melina T.",
year = "2014",
abstract = "Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.",
publisher = "Hindawi Limited",
journal = "Biomed research international",
title = "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions",
volume = "2014",
pages = "64930",
doi = "10.1155/2014/364930"
}

Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Lucic-Skorić, M., Filipović, J.,& Kalagasidis-Krušić, M. T.. (2014). Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed research international
Hindawi Limited., 2014, 64930.
https://doi.org/10.1155/2014/364930

Milašinović N, Knežević-Jugović Z, Milosavljević N, Lucic-Skorić M, Filipović J, Kalagasidis-Krušić MT. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed research international. 2014;2014:64930.
doi:10.1155/2014/364930 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lucic-Skorić, Marija, Filipović, Jovanka, Kalagasidis-Krušić, Melina T., "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions" in Biomed research international, 2014 (2014):64930,
https://doi.org/10.1155/2014/364930 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Jakovetić, Sonja
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lucić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis-Krušić, Melina T.
PY  - 2014
UR  - http://jakov.kpu.edu.rs/handle/123456789/589
AB  - This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Hindawi Publishing Corp, New York
T2  - Scientific world journal
T1  - Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel
DO  - 10.1155/2014/142123
ER  - 

@article{
author = "Milašinović, Nikola and Jakovetić, Sonja and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lucić, Marija and Filipović, Jovanka and Kalagasidis-Krušić, Melina T.",
year = "2014",
abstract = "This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Hindawi Publishing Corp, New York",
journal = "Scientific world journal",
title = "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel",
doi = "10.1155/2014/142123"
}

Milašinović, N., Jakovetić, S., Knežević-Jugović, Z., Milosavljević, N., Lucić, M., Filipović, J.,& Kalagasidis-Krušić, M. T.. (2014). Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific world journal
Hindawi Publishing Corp, New York..
https://doi.org/10.1155/2014/142123

Milašinović N, Jakovetić S, Knežević-Jugović Z, Milosavljević N, Lucić M, Filipović J, Kalagasidis-Krušić MT. Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific world journal. 2014;.
doi:10.1155/2014/142123 .

Milašinović, Nikola, Jakovetić, Sonja, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lucić, Marija, Filipović, Jovanka, Kalagasidis-Krušić, Melina T., "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel" in Scientific world journal (2014),
https://doi.org/10.1155/2014/142123 . .

TY  - JOUR
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Đorđević, Verica B.
AU  - Kalagasidis-Krušić, Melina T.
AU  - Knežević-Jugović, Zorica
AU  - Nedović, Viktor
AU  - Bugarski, Branko M.
PY  - 2014
UR  - http://jakov.kpu.edu.rs/handle/123456789/577
AB  - In this work chitosan microbeads were prepared by emulsion technique and loaded with thyme polyphenols by diffusion from an external aqueous solution of Thymus serpyllum L. The effects of concentrations of chitosan (1.5-3% (w/v)) and GA (glutaraldehyde) (0.1-0.4% (v/v)), as a crosslinking agent on the main properties of microbeads were assessed. The obtained microgel beads from similar to 220 to similar to 790 mu m in diameter were exposed to controlled drying process at air (at 37 degrees C) after which they contracted to irregular shapes (similar to 70-230 mu m). The loading of dried microbeads with polyphenols was achieved by swelling in the acidic medium. The swelling rate of microbeads decreased with the increase in GA concentration. Upon this rehydration, thyme polypheriols were effectively encapsulated (active load of 66-114mg GAEg(beads)(-1)) and the microbeads recovered a spherical shape. Both, the increase in the amount of the crosslinking agent and the presence of polyphenols, contributed to a more pronounced surface roughness of microbeads. The release of encapsulated polyphenols in simulated gastrointestinal fluids was prolonged to 3 h.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate polymers
T1  - Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols
VL  - 111
SP  - 901
EP  - 907
DO  - 10.1016/j.carbpol.2014.05.053
ER  - 

@article{
author = "Trifković, Kata T. and Milašinović, Nikola and Đorđević, Verica B. and Kalagasidis-Krušić, Melina T. and Knežević-Jugović, Zorica and Nedović, Viktor and Bugarski, Branko M.",
year = "2014",
abstract = "In this work chitosan microbeads were prepared by emulsion technique and loaded with thyme polyphenols by diffusion from an external aqueous solution of Thymus serpyllum L. The effects of concentrations of chitosan (1.5-3% (w/v)) and GA (glutaraldehyde) (0.1-0.4% (v/v)), as a crosslinking agent on the main properties of microbeads were assessed. The obtained microgel beads from similar to 220 to similar to 790 mu m in diameter were exposed to controlled drying process at air (at 37 degrees C) after which they contracted to irregular shapes (similar to 70-230 mu m). The loading of dried microbeads with polyphenols was achieved by swelling in the acidic medium. The swelling rate of microbeads decreased with the increase in GA concentration. Upon this rehydration, thyme polypheriols were effectively encapsulated (active load of 66-114mg GAEg(beads)(-1)) and the microbeads recovered a spherical shape. Both, the increase in the amount of the crosslinking agent and the presence of polyphenols, contributed to a more pronounced surface roughness of microbeads. The release of encapsulated polyphenols in simulated gastrointestinal fluids was prolonged to 3 h.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate polymers",
title = "Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols",
volume = "111",
pages = "901-907",
doi = "10.1016/j.carbpol.2014.05.053"
}

Trifković, K. T., Milašinović, N., Đorđević, V. B., Kalagasidis-Krušić, M. T., Knežević-Jugović, Z., Nedović, V.,& Bugarski, B. M.. (2014). Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols. in Carbohydrate polymers
Elsevier Sci Ltd, Oxford., 111, 901-907.
https://doi.org/10.1016/j.carbpol.2014.05.053

Trifković KT, Milašinović N, Đorđević VB, Kalagasidis-Krušić MT, Knežević-Jugović Z, Nedović V, Bugarski BM. Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols. in Carbohydrate polymers. 2014;111:901-907.
doi:10.1016/j.carbpol.2014.05.053 .

Trifković, Kata T., Milašinović, Nikola, Đorđević, Verica B., Kalagasidis-Krušić, Melina T., Knežević-Jugović, Zorica, Nedović, Viktor, Bugarski, Branko M., "Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols" in Carbohydrate polymers, 111 (2014):901-907,
https://doi.org/10.1016/j.carbpol.2014.05.053 . .

TY  - JOUR
AU  - Milašinović, Veljko
AU  - Milašinović, Nikola
PY  - 2012
UR  - http://jakov.kpu.edu.rs/handle/123456789/468
AB  - Technical forensics is a very broad scientific field. This paper reports one of its aspects, which refer to the testing of electrical conductors following a fire in order to identify the real cause of its appearance, namely providing information whether or not the electric cord damage caused the fire and to offer contribution to the authorities. For this purpose, sectional tests of copper electric cord were conducted using microstructure optical microscope as well as X-ray diffraction analysis. The sample preparation process has been also described. The interpretation of the obtained results with the adopted conclusions based on the above-mentioned tests was presented. Based on these analyses, it was concluded that there was a local melting of the conductors influenced by high temperatures achieved in oxygen atmosphere, and that melting was apparent within all samples.
AB  - Tehnička forenzika je jako široka naučna oblast. U ovom radu će se govoriti o jednom njenom segmentu koji se odnosi na ispitivanje električnih provodnika nakon požara, u cilju otkrivanja njegovog stvarnog uzroka, tj. prihvatanja ili odbacivanja kvara na elektoinstalacijama kao uzroka požara i pribavljanja informacija istražnim organima za rašavanje postavljenog zadatka. U tu svrhu su izvršena mikrostrukturna ispitivanja preseka bakarne žice korišćenjem optičkog mikroskopa i rendgenostrukturna analiza istih. Opisan je, takođe, i proces pripreme uzoraka kao i tumačenje dobijenih rezultata sa zaključcima donesenim na osnovu pomenutih rezultata ispitivanja. Na osnovu urađenih analiza je zaključeno da je došlo do lokalnog topljenja provodnika pod dejstvom visoke temperature u atmosferi kiseonika, a topljenje je uočeno kod svih ispitivanih uzoraka.
PB  - Kriminalističko- policijska akademija, Beograd
T2  - Nauka, bezbednost, policija
T1  - The technology of forensic examination of burned copper cord
T1  - Tehnologija forenzičkog ispitivanja bakarne žice nakon požara
VL  - 17
IS  - 3
SP  - 87
EP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_jakov_468
ER  - 

@article{
author = "Milašinović, Veljko and Milašinović, Nikola",
year = "2012",
abstract = "Technical forensics is a very broad scientific field. This paper reports one of its aspects, which refer to the testing of electrical conductors following a fire in order to identify the real cause of its appearance, namely providing information whether or not the electric cord damage caused the fire and to offer contribution to the authorities. For this purpose, sectional tests of copper electric cord were conducted using microstructure optical microscope as well as X-ray diffraction analysis. The sample preparation process has been also described. The interpretation of the obtained results with the adopted conclusions based on the above-mentioned tests was presented. Based on these analyses, it was concluded that there was a local melting of the conductors influenced by high temperatures achieved in oxygen atmosphere, and that melting was apparent within all samples., Tehnička forenzika je jako široka naučna oblast. U ovom radu će se govoriti o jednom njenom segmentu koji se odnosi na ispitivanje električnih provodnika nakon požara, u cilju otkrivanja njegovog stvarnog uzroka, tj. prihvatanja ili odbacivanja kvara na elektoinstalacijama kao uzroka požara i pribavljanja informacija istražnim organima za rašavanje postavljenog zadatka. U tu svrhu su izvršena mikrostrukturna ispitivanja preseka bakarne žice korišćenjem optičkog mikroskopa i rendgenostrukturna analiza istih. Opisan je, takođe, i proces pripreme uzoraka kao i tumačenje dobijenih rezultata sa zaključcima donesenim na osnovu pomenutih rezultata ispitivanja. Na osnovu urađenih analiza je zaključeno da je došlo do lokalnog topljenja provodnika pod dejstvom visoke temperature u atmosferi kiseonika, a topljenje je uočeno kod svih ispitivanih uzoraka.",
publisher = "Kriminalističko- policijska akademija, Beograd",
journal = "Nauka, bezbednost, policija",
title = "The technology of forensic examination of burned copper cord, Tehnologija forenzičkog ispitivanja bakarne žice nakon požara",
volume = "17",
number = "3",
pages = "87-96",
url = "https://hdl.handle.net/21.15107/rcub_jakov_468"
}

Milašinović, V.,& Milašinović, N.. (2012). The technology of forensic examination of burned copper cord. in Nauka, bezbednost, policija
Kriminalističko- policijska akademija, Beograd., 17(3), 87-96.
https://hdl.handle.net/21.15107/rcub_jakov_468

Milašinović V, Milašinović N. The technology of forensic examination of burned copper cord. in Nauka, bezbednost, policija. 2012;17(3):87-96.
https://hdl.handle.net/21.15107/rcub_jakov_468 .

Milašinović, Veljko, Milašinović, Nikola, "The technology of forensic examination of burned copper cord" in Nauka, bezbednost, policija, 17, no. 3 (2012):87-96,
https://hdl.handle.net/21.15107/rcub_jakov_468 .