Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method
Samo za registrovane korisnike
2016
Autori
Milašinović, NikolaCalija, Bojan
Vidović, Bojana
Crevar-Sakač, Milkica
Vujić, Zorica
Knežević-Jugović, Zorica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel co...mposition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.
Ključne reči:
Alpha-lipoic acid / Chitosan / Microbeads / Inverse emulsion method / Sustained releaseIzvor:
Journal of the Taiwan institute of chemical engineers, 2016, 60, 106-112Izdavač:
- Elsevier Science Bv, Amsterdam
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-46010)
- Razvoj mikro- i nanosistema kao nosača za lekove sa antiinflamatornim delovanjem i metoda za njihovu karakterizaciju (RS-34031)
DOI: 10.1016/j.jtice.2015.10.037
ISSN: 1876-1070