Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method
Authorized Users Only
2016
Authors
Milašinović, NikolaCalija, Bojan
Vidović, Bojana
Crevar-Sakač, Milkica
Vujić, Zorica
Knežević-Jugović, Zorica
Article (Published version)
Metadata
Show full item recordAbstract
The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel co...mposition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.
Keywords:
Alpha-lipoic acid / Chitosan / Microbeads / Inverse emulsion method / Sustained releaseSource:
Journal of the Taiwan institute of chemical engineers, 2016, 60, 106-112Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Development of micro- and nanosystems as carriers for drugs with anti-inflammatory effect and methods for their characterization (RS-MESTD-Technological Development (TD or TR)-34031)
DOI: 10.1016/j.jtice.2015.10.037
ISSN: 1876-1070
WoS: 000371937900011
Scopus: 2-s2.0-84958745685
Collections
Institution/Community
JakovTY - JOUR AU - Milašinović, Nikola AU - Calija, Bojan AU - Vidović, Bojana AU - Crevar-Sakač, Milkica AU - Vujić, Zorica AU - Knežević-Jugović, Zorica PY - 2016 UR - http://jakov.kpu.edu.rs/handle/123456789/735 AB - The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations. PB - Elsevier Science Bv, Amsterdam T2 - Journal of the Taiwan institute of chemical engineers T1 - Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method VL - 60 SP - 106 EP - 112 DO - 10.1016/j.jtice.2015.10.037 ER -
@article{ author = "Milašinović, Nikola and Calija, Bojan and Vidović, Bojana and Crevar-Sakač, Milkica and Vujić, Zorica and Knežević-Jugović, Zorica", year = "2016", abstract = "The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of the Taiwan institute of chemical engineers", title = "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method", volume = "60", pages = "106-112", doi = "10.1016/j.jtice.2015.10.037" }
Milašinović, N., Calija, B., Vidović, B., Crevar-Sakač, M., Vujić, Z.,& Knežević-Jugović, Z.. (2016). Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan institute of chemical engineers Elsevier Science Bv, Amsterdam., 60, 106-112. https://doi.org/10.1016/j.jtice.2015.10.037
Milašinović N, Calija B, Vidović B, Crevar-Sakač M, Vujić Z, Knežević-Jugović Z. Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan institute of chemical engineers. 2016;60:106-112. doi:10.1016/j.jtice.2015.10.037 .
Milašinović, Nikola, Calija, Bojan, Vidović, Bojana, Crevar-Sakač, Milkica, Vujić, Zorica, Knežević-Jugović, Zorica, "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method" in Journal of the Taiwan institute of chemical engineers, 60 (2016):106-112, https://doi.org/10.1016/j.jtice.2015.10.037 . .