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dc.creatorDay, James R.
dc.creatorDavid, Anu
dc.creatorKim, Jiwon
dc.creatorFarkash, Evan A.
dc.creatorCascalho, Marilia
dc.creatorMilašinović, Nikola
dc.creatorShikanov, Ariella
dc.date.accessioned2019-03-31T23:04:30Z
dc.date.available2019-12-12
dc.date.issued2018
dc.identifier.issn1742-7061
dc.identifier.urihttp://jakov.kpu.edu.rs/handle/123456789/892
dc.description.abstractPoly(ethylene glycol) (PEG) can be functionalized and modified with various moieties allowing for a multitude of cross-linking chemistries. Here, we investigate how vinyl sulfone, acrylate, and maleimide functional end groups affect hydrogel formation, physical properties, viability of encapsulated cells, post polymerization modification, and inflammatory response of the host. We have shown that PEG-VS hydro gels, in the presence of a co-monomer, N-vinyl-2-pyrrolidone (NVP), form more efficiently than PEG-Ac and PEG-Mal hydrogels, resulting in superior physical properties after 6 min of ultraviolet light exposure. PEG-VS hydrogels exhibited hydrolytic stability and non-fouling characteristics, as well as the ability to be modified with biological motifs, such as RGD, after polymerization. Additionally, unmodified PEG-VS hydrogels resulted in lesser inflammatory response, cellular infiltration, and macrophage recruitment after implantation for 28 days in mice. These findings show that altering the end group chemistry of PEG macromer impacts characteristics of the photo-polymerized network. We have developed a tunable non-degradable PEG system that is conducive for cell or tissue encapsulation and evokes a minimal inflammatory response, which could be utilized for future immunoisolation applications. Statement of Significance The objective of this study was to develop a tunable non-degradable PEG system that is conducive for encapsulation and evokes a minimal inflammatory response, which could be utilized for immunoisoladon applications. This study has demonstrated that reactive functional groups of the PEG macromers impact free radical mediated network formation. Here, we show PEG-VS hydrogels meet the design criteria for an immunoisolating device as PEG-VS hydrogels form efficiently via photo-polymerization, impacting bulk properties, was stable in physiological conditions, and elicited a minimal inflammatory response. Further, NVP can be added to the precursor solution to expedite the cross-linking process without impacting cellular response upon encapsulation. These findings present an additional approach/chemistry to encapsulate cells or tissue for immunoisolation applications.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationNational Institute of Biomedical Imaging and Bioengineering [R01 EB022033]
dc.relationHartwell Foundation [14PAF00981]
dc.relationNational Science Foundation [DGE 1256260]
dc.relationRonald and Eileen Weiser Center for Europe and Eurasia
dc.relationNIAD [K23AI108951]
dc.rightsembargoedAccess
dc.sourceActa biomaterialia
dc.subjectPoly(ethylene glycol)en
dc.subjectPhoto-polymerizationen
dc.subjectFunctional groupsen
dc.subjectRGD modificationen
dc.subjectInflammatory responseen
dc.titleThe impact of functional groups of poly(ethylene glycol) macromers on the physical properties of photo-polymerized hydrogels and the local inflammatory response in the hosten
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractМилашиновић, Никола
dc.citation.volume67
dc.citation.spage42
dc.citation.epage52
dc.citation.other67: 42-52
dc.citation.rankaM21
dc.identifier.doi10.1016/j.actbio.2017.12.007
dc.identifier.scopus2-s2.0-85039447172
dc.identifier.wos000424853600004
dc.type.versionacceptedVersion


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